PXD007872

PXD007872 is an original dataset announced via ProteomeXchange.

Title	MELK promotes melanoma growth by stimulating the NF-B pathway
Description	Melanoma accounts for over 80% of skin cancer-related deaths and current therapies provide only short-term benefit to patients. Here, we show in melanoma cells that maternal embryonic leucine zipper kinase (MELK) is transcriptionally upregulated by the MAP kinase pathway via transcription factor E2F1. MELK knockdown or pharmacological inhibition blocked melanoma growth and enhanced the effectiveness of BRAFV600E inhibitor against melanoma cells. To identify mediators of MELK function, we performed stable isotope labeling with amino acids in cell culture (SILAC) and identified 469 proteins that had downregulated phosphorylation after MELK inhibition. Remarkably, 139 of these proteins were previously reported as substrates of BRAF or MEK, demonstrating that MELK is an important downstream mediator of the MAPK pathway. Furthermore, we show that MELK promotes melanoma growth by activating NF-B pathway activity via Sequestosome 1 (SQSTM1/p62). Collectively, these results underpin an important role for MELK in melanoma growth, downstream of the MAPK pathway.
HostingRepository	PRIDE
AnnounceDate	2017-12-07
AnnouncementXML	Submission_2017-12-07_03:37:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	narendra wajapeyee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-29 05:37:16	ID requested
⏵ 1	2017-12-07 03:37:52	announced

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: Human, MELK

Narendra Wajapayee
contact affiliation	Pathology Department; Yale University
contact email	narendra.wajapayee@yale.edu
lab head
narendra wajapeyee
contact affiliation	Yale University
contact email	narendra.wajapeyee@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD007872

PRIDE project URI

• PRIDE
  1. PXD007872
     1. Label: PRIDE project
     2. Name: MELK promotes melanoma growth by stimulating the NF-B pathway