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PXD007872

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-12-07
  • AnnouncementXML: Submission_2017-12-07_03:37:51.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: narendra wajapeyee
  • Title: MELK promotes melanoma growth by stimulating the NF-B pathway
  • Description: Melanoma accounts for over 80% of skin cancer-related deaths and current therapies provide only short-term benefit to patients. Here, we show in melanoma cells that maternal embryonic leucine zipper kinase (MELK) is transcriptionally upregulated by the MAP kinase pathway via transcription factor E2F1. MELK knockdown or pharmacological inhibition blocked melanoma growth and enhanced the effectiveness of BRAFV600E inhibitor against melanoma cells. To identify mediators of MELK function, we performed stable isotope labeling with amino acids in cell culture (SILAC) and identified 469 proteins that had downregulated phosphorylation after MELK inhibition. Remarkably, 139 of these proteins were previously reported as substrates of BRAF or MEK, demonstrating that MELK is an important downstream mediator of the MAPK pathway. Furthermore, we show that MELK promotes melanoma growth by activating NF-B pathway activity via Sequestosome 1 (SQSTM1/p62). Collectively, these results underpin an important role for MELK in melanoma growth, downstream of the MAPK pathway.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: phosphorylated residue
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-09-29 05:37:16ID requested
12017-12-07 03:37:52announced

Publication List

  1. Dataset with its publication pending

Keyword List

  1. curator keyword: Biological, Biomedical
  2. submitter keyword: Human, MELK

Contact List

    Narendra Wajapayee
    • contact affiliation: Pathology Department; Yale University
    • contact email: narendra.wajapayee@yale.edu
    • lab head:
    narendra wajapeyee
    • contact affiliation: Yale University
    • contact email: narendra.wajapeyee@yale.edu
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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