PXD007866

PXD007866 is an original dataset announced via ProteomeXchange.

Title	Major Histocompatibility Complex I of swine respiratory cells present conserved regions of influenza proteins
Description	Swine influenza virus (SIV) is a prevalent respiratory pathogen in pigs that has deleterious consequences to animal health, production, and public health. Pigs represent an important reservoir for influenza as well as a potential mixing vessel for novel gene reassortments. Despite the central role of the pig in the 2009 pandemic and 2012 variant H3N2 outbreak, much remains unknown about the impact of swine immunity on SIV transmission, pathogenesis, and evolution. An incomplete understanding of interactions between the porcine immune system and SIV has hindered the development of new diagnostic tools and CD8+ T cell influenza epitope based vaccines. In order to address this gap in knowledge, we identified swine leukocyte antigen (SLA) restricted influenza virus peptides presented by swine respiratory epithelial cells using an immunoproteomics approach. The majority of MHC associated peptides belonged to matrix 1, nucleocapsid, and nonstructural 1 proteins. Specific epitopes, such as M1229-242, NS177-89, and NP417-426, may have value in epitope based vaccines. Future investigations examining the potential cross-reactive nature of these peptides are needed to confirm antigen recognition by cytotoxic T lymphocytes and utility as vaccine candidates.
HostingRepository	PRIDE
AnnounceDate	2018-02-28
AnnouncementXML	Submission_2018-02-28_05:57:40.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007866
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Elise Lamont
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	Ammonia-loss; Dehydrated; Oxidation; Acetyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-28 02:32:53	ID requested
⏵ 1	2018-02-28 05:57:41	announced

Lamont EA, Poulin E, Sreevatsan S, Cheeran MC, Major histocompatibility complex I of swine respiratory cells presents conserved regions of influenza proteins. J Gen Virol, 99(3):303-308(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: influenza, flu, swine, pigs, MHC, peptides

Maxim Cheeran
contact affiliation	225, Veterinary Medical Center University of Minnesota 1365, Gortner Avenue St. Paul, MN 55108
contact email	cheeran@umn.edu
lab head
Elise Lamont
contact affiliation	University of Minnesota
contact email	lamo0062@umn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/02/PXD007866

PRIDE project URI

• PRIDE
  1. PXD007866
     1. Label: PRIDE project
     2. Name: Major Histocompatibility Complex I of swine respiratory cells present conserved regions of influenza proteins