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PXD007862

PXD007862 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBIOSITe: A novel method for direct detection of site specific biotinylation
DescriptionProximity-dependent biotinylation methods, such as those mediated by BioID and APEX, are being widely adopted for studying in vivo protein-protein interaction dynamics and subcellular structures. However, these along with other biotin-based technologies are severely dependent on avidin family proteins for the capture of biotinylated proteins. While the extremely high affinity of the avidin-biotin interaction nearly guarantees the capture of biotinylated proteins from a sample, it also results in an inability to recover the key species of interest: the biotin-modified peptide. To overcome this limitation, we have developed a novel strategy that instead relies on an immunoaffinity-based capture of biotinylated peptides for downstream LC-MS/MS analysis. We refer to this approach as Biotinylation Site Identification Technology (BioSITe), since it enables the direct detection of site-specific biotinylation, which substantially increases the sensitivity and specificity of data offered by proximity-dependent biotinylation methods. Using isotopically labeled biotin, we also demonstrate that this method can be used for differential analysis. Overall, we anticipate this method to replace the current methods used for detection of biotinylation sites and other applications including those employing any molecule probe for enrichment of specific classes of proteins or post-translational modifications.
HostingRepositoryPRIDE
AnnounceDate2017-12-19
AnnouncementXMLSubmission_2017-12-19_02:18:18.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAkhilesh Pandey
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListbiotinylated residue
InstrumentOrbitrap Fusion Lumos; Orbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-09-28 01:21:37ID requested
12017-12-19 02:18:19announced
Publication List
Kim DI, Cutler JA, Na CH, Reckel S, Renuse S, Madugundu AK, Tahir R, Goldschmidt HL, Reddy KL, Huganir RL, Wu X, Zachara NE, Hantschel O, Pandey A, BioSITe: A Method for Direct Detection and Quantitation of Site-Specific Biotinylation. J Proteome Res, 17(2):759-769(2018) [pubmed]
Keyword List
curator keyword: Technical
submitter keyword: BioID, Biotinylation, IP, BCR ABL, peptide, mass spectrometry, proximity dependent biotinylation, BioSITe,
Contact List
Akhilesh Pandey
contact affiliationMcKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
contact emailpandey@jhmi.edu
lab head
Akhilesh Pandey
contact affiliationJohns Hopkins University
contact emailpandey@jhmi.edu
dataset submitter
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