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PXD007860

PXD007860 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterizing T-cell immunopeptidomes
DescriptionImunopeptidomes promise novel surface markers as ideal targets for immunotherapies, but their characterization by mass spectrometry (MS) remains challenging. Until recently, cell numbers exceeding 1.0xE9 were needed for reasonably deep immunopeptidome surveys of at least 1,000 HLA ligands. Such limitation in analytical sensitivity necessarily constrains the types of clinical specimens that can evaluated. To evaluate the feasibility of immunopeptidome profiling from limiting cell amounts, primary purified human regulatory (Treg) and conventional T cells (Tconv) were compared, evaluating the relative immunopeptide recovery from T cells isolated from individual healthy donors (N=5) versus pools (N=2 pools from 13 donors). Two donors’ Tconv were subsequently stimulated with CD3/CD28 and IL-2 and compared to resting Tconv. The combined T-cell immunopeptide dataset reported here contains over 13,000 unique peptides derived from over 5,000 proteins. In comparison to protein expression, T cell immunopeptidomes revealed complementary aspects of T cell biology, while pointing to HLA ligands specific for distinct T cell subsets (e.g., FOXP3, CD5 and WNK1). Taken together, these findings have implications for understanding conditions required to induce therapeutic immune responses and may open up new avenues for tuning Treg suppressive functions to treat cancer.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:40:46.278.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNiclas Olsson
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; monohydroxylated residue; deamidated residue
InstrumentOrbitrap Fusion Lumos; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-09-28 01:17:59ID requested
12018-03-08 06:09:23announced
22024-10-22 04:40:51announced2024-10-22: Updated project metadata.
Publication List
Olsson N, Schultz LM, Zhang L, Khodadoust MS, Narayan R, Czerwinski DK, Levy R, Elias JE, T-Cell Immunopeptidomes Reveal Cell Subtype Surface Markers Derived From Intracellular Proteins. Proteomics, 18(12):e1700410(2018) [pubmed]
10.1002/pmic.201700410;
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Human, Antigen presentation, T cell, MHC, Orbitrap
Contact List
Joshua E Elias
contact affiliationDepartment of Chemical & Systems Biology, Stanford University, USA
contact emailjosh.elias@stanford.edu
lab head
Niclas Olsson
contact affiliationCalico Life Sciences
contact emailnic.e.olsson@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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