PXD007858

PXD007858 is an original dataset announced via ProteomeXchange.

Title	Asc1p-dependent phosphorylation of Ubp3p-associated Bre5p
Description	The phosphorylation of Bre5p was analyzed in ASC1 wild-type and Δasc1 cells. Bre5p was co-purified from cell extracts with its GFP-tagged interaction partner Ubp3p in GFP-trap experiments. Trapped proteins were in-gel digested with trypsin and analyzed with the Q Exactive HF (Thermo Scientific). Protein and phospho-peptide identification and calculation of LFQ intensities were done with the MaxQuant software. For validation of phospho-peptides/-sites single ion monitoring by tSIM analysis was performed.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:40:41.136.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Valerius
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-27 05:08:44	ID requested
1	2017-10-09 03:15:37	announced
⏵ 2	2024-10-22 04:40:43	announced	2024-10-22: Updated project metadata.

Opitz N, Schmitt K, Hofer-Pretz V, Neumann B, Krebber H, Braus GH, Valerius O, (RACK1) Microenvironment at the Head Region of the 40S Ribosome with Quantitative BioID in Yeast. Mol Cell Proteomics, 16(12):2199-2218(2017) [pubmed]

10.1074/mcp.m116.066654;

curator keyword: Biological

submitter keyword: Bre5, phosphorylation, Ubp3,Saccharomyces cerevisiae, Asc1/RACK1

Oliver Valerius
contact affiliation	Georg-August-University Göttingen Department of Molecular Microbiology and Genetics Institute of Microbiology and Genetics
contact email	ovaleri@gwdg.de
lab head
Oliver Valerius
contact affiliation	Georg-August-Universität Göttingen
contact email	ovaleri@gwdg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007858
     1. Label: PRIDE project
     2. Name: Asc1p-dependent phosphorylation of Ubp3p-associated Bre5p