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PXD007858

PXD007858 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAsc1p-dependent phosphorylation of Ubp3p-associated Bre5p
DescriptionThe phosphorylation of Bre5p was analyzed in ASC1 wild-type and Δasc1 cells. Bre5p was co-purified from cell extracts with its GFP-tagged interaction partner Ubp3p in GFP-trap experiments. Trapped proteins were in-gel digested with trypsin and analyzed with the Q Exactive HF (Thermo Scientific). Protein and phospho-peptide identification and calculation of LFQ intensities were done with the MaxQuant software. For validation of phospho-peptides/-sites single ion monitoring by tSIM analysis was performed.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:40:41.136.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOliver Valerius
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListphosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-09-27 05:08:44ID requested
12017-10-09 03:15:37announced
22024-10-22 04:40:43announced2024-10-22: Updated project metadata.
Publication List
10.1074/mcp.m116.066654;
Keyword List
curator keyword: Biological
submitter keyword: Bre5, phosphorylation, Ubp3,Saccharomyces cerevisiae, Asc1/RACK1
Contact List
Oliver Valerius
contact affiliationGeorg-August-University Göttingen Department of Molecular Microbiology and Genetics Institute of Microbiology and Genetics
contact emailovaleri@gwdg.de
lab head
Oliver Valerius
contact affiliationGeorg-August-Universität Göttingen
contact emailovaleri@gwdg.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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