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PXD007857

PXD007857 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBioID reveals novel proteins of the Plasmodium parasitophorous vacuole membrane
DescriptionDuring their development within the vertebrate host, Plasmodium parasites infect hepatocytes and red blood cells. Within these cells, parasites are surrounded by a parasitophorous vacuole membrane (PVM). The PVM plays an essential role for the interaction of parasites with their host cells, however only a limited number of proteins of this membrane have been identified so far. This is partially because a systematic proteomic analysis of the PVM’s protein content has been difficult in the past, due to difficulties to separate the PVM from other membranes such as the parasite plasma membrane. In this study, we adapted the BioID technique to in vitro cultivated Plasmodium berghei blood stage parasites and utilized the promiscuous biotin ligase BirA* fused to the PVM-resident exported protein 1 to biotinylate proteins of the PVM. These we further processed by affinity purification, liquid chromatography based mass spectrometry (LC-MS/MS) and label-free quantitation leading to a list of 61 known and candidate PVM proteins. Seven proteins were analyzed further during blood and liver stage development. This resulted in the identification of three novel PVM proteins, which were the serine/threonine protein phosphatase UIS2 and two conserved Plasmodium proteins with unknown functions (PBANKA_0519300 and PBANKA_0509000). In conclusion, our study expands the number of known PVM proteins and experimentally validates BioID as a powerful method to screen for novel constituents of specific cellular compartments in P. berghei.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:42:45.088.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDamaris Bausch-Fluck
SpeciesList scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-09-27 04:25:51ID requested
12018-01-03 00:25:45announced
22018-01-04 01:34:43announcedUpdated project metadata.
32018-02-07 02:30:34announcedUpdated project metadata.
42024-10-22 04:42:52announced2024-10-22: Updated project metadata.
Publication List
10.1128/msphere.00522-17;
Schnider CB, Bausch-Fluck D, Br, ü, hlmann F, Heussler VT, Burda PC, Parasitophorous Vacuole Membrane. mSphere, 3(1):(2018) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: malaria, BioID,plasmodium berghei, plasma membrane
Contact List
Prof. Dr. Volker Heussler
contact affiliationUniversität Bern Institut für Zellbiologie Baltzerstrasse 4 CH-3012 Bern
contact emailvolker.heussler@izb.unibe.ch
lab head
Damaris Bausch-Fluck
contact affiliationInstitute of Molecular Systems Biology
contact emailbausch@imsb.biol.ethz.ch
dataset submitter
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Dataset FTP location
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PRIDE project URI
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