PXD007857

PXD007857 is an original dataset announced via ProteomeXchange.

Title	BioID reveals novel proteins of the Plasmodium parasitophorous vacuole membrane
Description	During their development within the vertebrate host, Plasmodium parasites infect hepatocytes and red blood cells. Within these cells, parasites are surrounded by a parasitophorous vacuole membrane (PVM). The PVM plays an essential role for the interaction of parasites with their host cells, however only a limited number of proteins of this membrane have been identified so far. This is partially because a systematic proteomic analysis of the PVM’s protein content has been difficult in the past, due to difficulties to separate the PVM from other membranes such as the parasite plasma membrane. In this study, we adapted the BioID technique to in vitro cultivated Plasmodium berghei blood stage parasites and utilized the promiscuous biotin ligase BirA* fused to the PVM-resident exported protein 1 to biotinylate proteins of the PVM. These we further processed by affinity purification, liquid chromatography based mass spectrometry (LC-MS/MS) and label-free quantitation leading to a list of 61 known and candidate PVM proteins. Seven proteins were analyzed further during blood and liver stage development. This resulted in the identification of three novel PVM proteins, which were the serine/threonine protein phosphatase UIS2 and two conserved Plasmodium proteins with unknown functions (PBANKA_0519300 and PBANKA_0509000). In conclusion, our study expands the number of known PVM proteins and experimentally validates BioID as a powerful method to screen for novel constituents of specific cellular compartments in P. berghei.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:42:45.088.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Damaris Bausch-Fluck
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-27 04:25:51	ID requested
1	2018-01-03 00:25:45	announced
2	2018-01-04 01:34:43	announced	Updated project metadata.
3	2018-02-07 02:30:34	announced	Updated project metadata.
⏵ 4	2024-10-22 04:42:52	announced	2024-10-22: Updated project metadata.

10.1128/msphere.00522-17;

Schnider CB, Bausch-Fluck D, Br, ü, hlmann F, Heussler VT, Burda PC, Parasitophorous Vacuole Membrane. mSphere, 3(1):(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: malaria, BioID,plasmodium berghei, plasma membrane

Prof. Dr. Volker Heussler
contact affiliation	Universität Bern Institut für Zellbiologie Baltzerstrasse 4 CH-3012 Bern
contact email	volker.heussler@izb.unibe.ch
lab head
Damaris Bausch-Fluck
contact affiliation	Institute of Molecular Systems Biology
contact email	bausch@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD007857

PRIDE project URI

• PRIDE
  1. PXD007857
     1. Label: PRIDE project
     2. Name: BioID reveals novel proteins of the Plasmodium parasitophorous vacuole membrane