PXD007842

PXD007842 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of biological fluids of sleeping sickness patients
Description	Background: Human African trypanosomiasis (HAT), also called sleeping sickness, is one of 20 in WHO’s list of ‘Neglected Tropical Diseases’, for which, less than 3,500 cases are reported each year. The disease is caused by the parasite Trypanosoma brucei. Even though the clinical symptoms are well described, physiological aspects of the disease remain unclear. Diagnosis of the disease is difficult, requiring a lumbar puncture to determine the disease stage and guide treatment. Identifying new markers for disease stage has been a challenge for many years. Methods and Findings: In our study, a liquid chromatography coupled with tandem mass spectrometry tandem approach (LC-MS/MS) provides a new way to establish the proteomic profile of patients in stages 1 and 2 of the disease. The biological fluids (serum, cerebrospinal fluid, urine and saliva) of three uninfected controls, three patients with stage 1 disease, and four patients with stage 2 disease, are analyzed. In addition, quantification of samples from 14 controls, 23 patients with stage 1 disease and 43 patients with stage 2 disease by ELISA completes this analysis, and highlights two potential new markers, neuroserpin and moesin, with the latter present in urine — an easily accessed fluid. Conclusion: These result suggest that quantifying proteins present in biological fluids by LC-MS/MS) could provide new biomarkers for the diagnosis and stage discrimination of HAT.
HostingRepository	PRIDE
AnnounceDate	2018-11-15
AnnouncementXML	Submission_2018-11-15_13:50:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut LEGER
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-26 02:07:45	ID requested
⏵ 1	2018-11-15 13:50:51	announced

Bonnet J, Garcia C, Leger T, Couquet MP, Vignoles P, Vatunga G, Ndung'u J, Boudot C, Bisser S, Courtioux B, Proteome characterization in various biological fluids of Trypanosoma brucei gambiense-infected subjects. J Proteomics, 196():150-161(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: trypanosoma, patients, LC-MS/MS, quantitative proteomics, urine, serum, CSF, saliva

Bertrand Courtioux
contact affiliation	UMR INSERM 1094 Neuroépidémiologie Tropicale Institut d'Epidémiologie Neurologique et de Neurologie Tropicale Faculté de Pharmacie 2, rue du Dr Marcland 87025 Limoges FRANCE
contact email	bertrand.courtioux@unilim.fr
lab head
Thibaut LEGER
contact affiliation	Institut Jacques Monod
contact email	thibaut.leger@ijm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD007842

PRIDE project URI

• PRIDE
  1. PXD007842
     1. Label: PRIDE project
     2. Name: Proteomics analysis of biological fluids of sleeping sickness patients