PXD007839

PXD007839 is an original dataset announced via ProteomeXchange.

Title	Human cardiac fibroblast membrane proteome
Description	Cardiac Fibroblasts (CFs) are one of the main cell populations in the heart and play important roles in tissue homeostasis and in myocardial fibrosis. The study of these cells has been hampered by the lack of reliable membrane markers: none of the antigens currently used for characterization and isolation of CFs is unique for this cell type. This issue has also raised doubts regarding a distinct identity of cardiac fibroblasts when comparing to other myocardium cell populations with similar morphologies. In this work, we report a comprehensive description and functional analysis of human CFs (hCFs) enriched membrane fraction proteome by advanced proteomic mass spectrometry-based tools. A total number of 1478 proteins were identified, including a 916 membrane proteins (62%). We also report on the identification of a set of 37 membrane proteins specific for hCFs by direct comparison with proteome lists of human mesenchymal stem cells (hMSCs), human dermal fibroblasts (hDFs) and human cardiac stem cells (hCSCs, hCSCs membrane proteome list is also available in this repository). The data reported in this work is a valuable source of information for further studies aiming at the discovery of a membrane molecular signature of hCFs.
HostingRepository	PRIDE
AnnounceDate	2018-05-11
AnnouncementXML	Submission_2018-05-11_08:12:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Sebastião
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-26 01:42:48	ID requested
⏵ 1	2018-05-11 08:12:39	announced

Sebasti, ã, o MJ, Pereira R, Serra M, Gomes-Alves P, Alves PM, Unveiling Human Cardiac Fibroblast Membrane Proteome. Proteomics, 18(13):e1700446(2018) [pubmed]

curator keyword: Biological

submitter keyword: human cardiac fibroblasts, myocardium, membrane proteins

Paula Marques Alves
contact affiliation	ITQB-NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. Da República, 2780-157 Oeiras, Portugal;iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901, Oeiras , Portugal
contact email	marques@ibet.pt
lab head
Maria Sebastião
contact affiliation	iBET/ ITQB-NOVA
contact email	msebastiao@itqb.unl.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD007839

PRIDE project URI

• PRIDE
  1. PXD007839
     1. Label: PRIDE project
     2. Name: Human cardiac fibroblast membrane proteome