PXD007836

PXD007836 is an original dataset announced via ProteomeXchange.

Title	PVY Coat Protein Interactome - In order to identify cellular proteins interacting with the coat protein (CP) of Potato virus Y (PVY), we used coimmunoprecipitation of GFP-CP in PVY-infected Nicotiana benthamiana plants. To be able to pull down non-abundant interactors and to stabilize transient interactions, we used the crosslinker dithiobis(succinimidyl propionate) (DSP). With this we were able to identify a total of 147 potential CP interactors.
Description	In order to identify cellular proteins interacting with the coat protein (CP) of Potato virus Y (PVY), we used coimmunoprecipitation of GFP-CP in PVY-infected Nicotiana benthamiana plants. To be able to pull down non-abundant interactors and to stabilize transient interactions, we used the crosslinker dithiobis(succinimidyl propionate) (DSP). With this we were able to identify a total of 147 potential CP interactors.
HostingRepository	PRIDE
AnnounceDate	2020-03-12
AnnouncementXML	Submission_2020-03-12_03:42:23.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007836
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christian Lamm
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-26 00:30:52	ID requested
1	2017-12-18 01:14:53	announced
⏵ 2	2020-03-12 03:42:25	announced	2020-03-12: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Potato Virus Y, PVY, Coat Protein, CP, DSP, Crosslinking, Nicotiana benthamiana

Uwe Sonnewald
contact affiliation	Division of Biochemistry, Department of Biology, Friedrich-Alexander University Erlangen-Nuremberg, Staudtstra??e 5, 91058 Erlangen
contact email	Uwe.Sonnewald@fau.de
lab head
Christian Lamm
contact affiliation	Friedrich-Alexander Universit??t Erlangen-N??rnberg
contact email	Christian.lamm@fau.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD007836

PRIDE project URI

• PRIDE
  1. PXD007836
     1. Label: PRIDE project
     2. Name: PVY Coat Protein Interactome - In order to identify cellular proteins interacting with the coat protein (CP) of Potato virus Y (PVY), we used coimmunoprecipitation of GFP-CP in PVY-infected Nicotiana benthamiana plants. To be able to pull down non-abundant interactors and to stabilize transient interactions, we used the crosslinker dithiobis(succinimidyl propionate) (DSP). With this we were able to identify a total of 147 potential CP interactors.