PXD007816

PXD007816 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Examination of matricellular fibrosis and wound healing in a model of pancreatic cancer progression
Description	Pancreatic ductal adenocarcinoma (PDAC) has the highest amount of stroma when compared to other solid tumors. It is now well known that the stroma plays an important role in supporting pancreatic cancer development and progression and this knowledge has led to the development of potential anti-stromal therapies, some of which have shown synergistic effect in combination with gemcitabine. Despite the acknowledgement of its importance, a granular view of how stromal composition and cross-linking change during the course of PDAC progression remains unfounded. Generally, the fibrosis that occurs in solid tumors appears to be driven primarily by increases in the abundance of fibrillar collagen, whose insolubility is determined by the formation of covalent intermolecular cross-links. Here we have used semi-quantitative proteomics and cross-linked amino acid analysis to characterize the composition and crosslinking status of normal pancreatic and PDAC stroma. In order to mimic the desmoplastic stroma observed in human disease, we utilized the genetically engineered KTC mouse model of PDAC (KrasLSL-G12D/+Tgfbr2flox/+Ptf1a-Cre). We found that highly fibrotic PDACs alter the solubility of their collagen-rich stroma through changes in the abundance of collagen cross-links and supports development of matricellular fibrosis associated with a wound healing response.
HostingRepository	MassIVE
AnnounceDate	2018-01-21
AnnouncementXML	Submission_2018-01-21_15:11:17.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alex Barrett
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Oxidation; Acetyl; Gln->pyro-Glu
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-21 16:18:53	ID requested
⏵ 1	2018-01-21 15:11:17	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: PDAC, solid tumor, extracellular matrix, matrisome, chemical digestion, proteomics, mass spectrometry, fibrosis

submitter keyword: PDAC, solid tumor, extracellular matrix, matrisome, chemical digestion, proteomics, mass spectrometry, fibrosis

Contact List

Kirk Hansen
contact affiliation	University of Colorado Anschutz Medical Campus
contact email	kirk.hansen@ucdenver.edu
lab head
Alex Barrett
contact affiliation	University of Colorado Anschutz Medical Campus
contact email	alexander.barrett@ucdenver.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000081553