PXD007800

PXD007800 is an original dataset announced via ProteomeXchange.

Title	Cytosolic protein Vms1 links ribosome quality control to mitochondrial and cellular homeostasis
Description	Eukaryotic cells have evolved extensive protein quality control mechanisms to remove faulty translation products. Here we show that yeast cells continually produce faulty mitochondrial polypeptides that stall on the ribosome during translation but are imported into the mitochondria. The cytosolic protein Vms1, together with the E3 ligase Ltn1, protects against the mitochondrial toxicity of these proteins and maintains cell viability under respiratory conditions. In the absence of these factors, stalled polypeptides aggregate after import and sequester critical mitochondrial chaperone and translation machinery. Aggregation depends on C-terminal alanyl/threonyl sequences (CAT-tails) that are attached to stalled polypeptides on 60S ribosomes by Rqc2. Vms1 binds to 60S ribosomes at the mitochondrial surface and antagonizes Rqc2, thereby facilitating import, impeding aggregation and directing aberrant polypeptides to intra-mitochondrial quality control. Vms1 is a key component of a rescue pathway for ribosome-stalled mitochondrial polypeptides that are inaccessible to ubiquitylation, due to coupling of translation and translocation.
HostingRepository	PRIDE
AnnounceDate	2017-10-27
AnnouncementXML	Submission_2017-11-08_00:06:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liang Zhao
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-20 07:54:44	ID requested
1	2017-10-27 06:28:44	announced
⏵ 2	2017-11-08 00:06:33	announced	Updated publication reference for PubMed record(s): 29107329.

Izawa T, Park SH, Zhao L, Hartl FU, Neupert W, Cytosolic Protein Vms1 Links Ribosome Quality Control to Mitochondrial and Cellular Homeostasis. Cell, 171(4):890-903.e18(2017) [pubmed]

curator keyword: Biological

submitter keyword: Vms1, Ltn1,Mitochondria, RQC

Walter Neupert
contact affiliation	1Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany 2Division of Cell Biology, Biomedical Center, Faculty of Medicine, University of Munich, Großhaderner Str. 9, 82152 Martinsried, Germany
contact email	neupert@med.uni-muenchen.de
lab head
Liang Zhao
contact affiliation	Max Planck Institute of Biochemistry
contact email	zhao@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD007800

PRIDE project URI

• PRIDE
  1. PXD007800
     1. Label: PRIDE project
     2. Name: Cytosolic protein Vms1 links ribosome quality control to mitochondrial and cellular homeostasis