PXD007794

PXD007794 is an original dataset announced via ProteomeXchange.

Title	Orthogonal interactome profiling of RNF41
Description	Ring finger protein 41 (RNF41) is an E3 ubiquitin ligase involved in the ubiquitination and degradation of many proteins such as ErbB3 receptors, BRUCE and parkin. Next to this, RNF41 regulates the intracellular trafficking of certain JAK-2 associated cytokine receptors by ubiquitinating and suppressing USP8 which in turn destabilizes the ESCRT-0 complex. To further elucidate the function of RNF41 we used different orthogonal approaches to reveal the RNF41 protein complex: Affinity Purification-Mass Spectrometry, BioID and Virotrap. We combined these results with known datasets for RNF41obtained with microarray MAPPIT and Y2H screens. By combining all these datasets , we provide a comprehensive high resolution interactome network. To further remove potential methodological artifacts from this network, we further distilled the data into a high confidence interactome map by retaining protein hits identified in two or more of the orthogonal methods. AP2S1, a novel RNF41 interaction partner, was selected from this high confidence interactome for further functional validation. We reveal a role for AP2S1 in leptin and LIF receptor signaling and show that RNF41 stabilizes and relocates AP2S1.
HostingRepository	PRIDE
AnnounceDate	2018-03-26
AnnouncementXML	Submission_2018-03-26_02:24:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sven Eyckerman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-19 07:21:38	ID requested
⏵ 1	2018-03-26 02:24:28	announced

Masschaele D, Wauman J, Vandemoortele G, De Sutter D, De Ceuninck L, Eyckerman S, Tavernier J, High-Confidence Interactome for RNF41 Built on Multiple Orthogonal Assays. J Proteome Res, 17(4):1348-1360(2018) [pubmed]

curator keyword: Biological

submitter keyword: human AP-MS Viortrap BioID

Sven Eyckerman
contact affiliation	VIB-UGent Center for Medical Biotechnology A. Baertsoenkaai 3 B-9000 Gent Belgium
contact email	sven.eyckerman@vib-ugent.be
lab head
Sven Eyckerman
contact affiliation	Medical Protein Research
contact email	sven.eyckerman@vib-ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007794
     1. Label: PRIDE project
     2. Name: Orthogonal interactome profiling of RNF41