PXD007779

PXD007779 is an original dataset announced via ProteomeXchange.

Title	Effect of acadesine and overexpression of the karyopherin Kap123 on nuclear abundance of yeast proteins
Description	Based on genetic and affinity chromatography experiments, the karyopherin Kap123 was identified as an important player in the cellular response to the drug acadesine (AICAR, 5′-phosphoribosyl-5-amino-4-imidazole carboxamide). Since Kap123 mediates nuclear import of multiple proteins we hypothesized that AICAR could affect nuclear trafficking. The effect of acadesine (AICAR) on nuclear trafficking was assayed by comparing protein composition of enriched nuclear extracts from yeast treated or not with AICAR by label-free quantitative analysis. Total protein extracts from the same cultures were also assayed by quantitative mass spectrometry. A parallel analysis was performed on yeast cells grown in the presence of AICAR and overexpressing or not Kap123 to identify those proteins that are enriched in the nuclear fraction when Kap123 was overexpressed.
HostingRepository	PRIDE
AnnounceDate	2018-11-23
AnnouncementXML	Submission_2018-11-23_04:04:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Claverol Claverol
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-18 05:50:41	ID requested
⏵ 1	2018-11-23 04:04:09	announced

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: karyopherin, AICAR, nucleus, yeast

Benoit Pinson
contact affiliation	University of Bordeaux, IBGC UMR 5095, F-33077 Bordeaux, France
contact email	pinson@ibgc.cnrs.fr
lab head
Claverol Claverol
contact affiliation	Plateforme Proteome
contact email	stephane.claverol@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD007779

PRIDE project URI

• PRIDE
  1. PXD007779
     1. Label: PRIDE project
     2. Name: Effect of acadesine and overexpression of the karyopherin Kap123 on nuclear abundance of yeast proteins