PXD007772

PXD007772 is an original dataset announced via ProteomeXchange.

Title	Functional genomics discovery in an emerging livestock parasite, the rumen fluke Calicophoron daubneyi; identifying shifts in rumen functionality.
Description	Diseases caused by parasitic flatworms of rumen tissues (paramphistomosis) are a significant threat to global food security as a cause of morbidity and mortality in ruminant livestock in subtropical and tropical climates. Calicophoron daubneyi is currently the only paramphistome species commonly infecting livestock species in temperate European climates. However, recorded incidences of C. daubneyi infection in European livestock have been increasing over the last decade. Whilst clinical paramphistomosis caused by adult worms is currently uncommon in Europe, fatalities have been reported in association with severe haemorrhagic enteritis resulting from the migration of immature paramphistomes. Large numbers of mature adults can reside in the rumen, yet to date, the impact on rumen fermentation, and consequently in productivity and economic management of infected livestock have not been resolved. Limited public available nucleotide and protein sequences for C. daubneyi underpin this lack of biological and economic understanding. Here we present for the first time a de novo assembled transcriptome, with functional annotations, for adult C. daubneyi, which provides a reference database for protein and nucleotide sequence identification to facilitate fundamental biology, anthelmintic, vaccine and diagnostics discoveries. This dataset identifies a number of genes potentially unique to C. daubneyi and, by comparison to an existing transcriptome for the related Paramphistomum cervi, identifies novel genes which may be unique to the paramphistome group of platyhelminthes. Additionally, we present the first coverage of the excretory/secretory and soluble somatic proteome profiles for adult C. daubneyi and identify the release of extracellular vesicles from adult C. daubneyi parasites during in vitro, ex-host culture. Finally, we have performed the first analysis of rumen fluke impacting upon rumen fermentation parameters using an in vitro gas production study resulting in a significant increase in propionate production.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_09:56:51.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007772
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Russ Morphew
SpeciesList	scientific name: Calicophoron daubneyi; NCBI TaxID: 300641;
ModificationList	Oxidation; Carbamidomethyl
Instrument	6550 iFunnel Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-18 05:25:27	ID requested
⏵ 1	2019-11-12 09:56:53	announced

Huson KM, Morphew RM, Allen NR, Hegarty MJ, Worgan HJ, Girdwood SE, Jones EL, Phillips HC, Vickers M, Swain M, Smith D, Kingston-Smith AH, Brophy PM, Polyomic tools for an emerging livestock parasite, the rumen fluke Calicophoron daubneyi

identifying shifts in rumen functionality. Parasit Vectors, 11(1):617(2018) [pubmed]

curator keyword: Biological

submitter keyword: Parasite

Rumen

Russell M Morphew
contact affiliation	IBERS, Aberystwyth University
contact email	rom@aber.ac.uk
lab head
Russ Morphew
contact affiliation	Aberystwyth University
contact email	rom@aber.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007772
     1. Label: PRIDE project
     2. Name: Functional genomics discovery in an emerging livestock parasite, the rumen fluke Calicophoron daubneyi; identifying shifts in rumen functionality.