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PXD007768

PXD007768 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSILAC-based proteomic approach to delineate molecular alterations associated with erlotinib resistance in head and neck squamous cell carcinoma
DescriptionAlthough Epidermal growth factor receptor (EGFR) is overexpressed in 90% of Head and neck squamous cell carcinoma (HNSCC) patients. Clinical trials with EGFR-targeted small molecule inhibitors such as erlotinib have shown a modest activity in recurrent or advanced HNSCC. To investigate the acquired mechanisms of erlotinib resistance we employed SILAC-based total proteomic analysis of an isogenic pair of erlotinib sensitive (SCC-S) and resistant (SCC-R) HNSCC cell line. This resulted in the identification of 5,427 proteins of which 532 proteins were overexpressed and 527 proteins were downregulated in SCC-R cells as compared to SCC-S cells (≥2 fold). Several proteins known to mediate erlotinib resistance in HNSCC and lung cancer were found to be dysregulated. Bioinformatics analysis of differentially expressed proteins showed enrichment of proteins involved in focal adhesion kinase (FAK) pathway downstream of EGFR. We identified CUB-domain containing protein 1 (CDCP1) and integrin β1 as upstream regulators of FAK signalling pathway to be overexpressed. We further demonstrated that CDCP1 and FAK can be targeted in combination as an alternative to erlotinib in HNSCC.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:40:59.551.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHarsha Gowda
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListisotope labeled residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-09-18 03:43:53ID requested
12019-12-18 08:18:00announced
22024-10-22 04:41:00announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41598-019-55208-5;
Jain AP, Patel K, Pinto S, Radhakrishnan A, Nanjappa V, Kumar M, Raja R, Patil AH, Kumari A, Manoharan M, Karunakaran C, Murugan S, Keshava Prasad TS, Chang X, Mathur PP, Kumar P, Gupta R, Gupta R, Khanna-Gupta A, Sidransky D, Chatterjee A, Gowda H, MAP2K1 is a potential therapeutic target in erlotinib resistant head and neck squamous cell carcinoma. Sci Rep, 9(1):18793(2019) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: head and neck cancer, mass spectrometry, signaling pathways, quantitative proteomics,EGFR-tyrosine kinase inhibitor resistance
Contact List
Harsha Gowda
contact affiliationInstitute of Bioinformatics , International Technology Park, Bangalore, India
contact emailharsha@ibioinformatics.org
lab head
Harsha Gowda
contact affiliationQIMR Berghofer Medical Research Institute
contact emailharsha@ibioinformatics.org
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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