PXD007764 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of Naegleria gruberi mitochondria |
Description | The formation of mitochondria has enabled eukaryotes to thrive and evolve into multitude of unicellular and multicellular species. The nature of the early mitochondria and its relationship to the ancestral host cell is still unknown. Using a phylogenomic approach we have discovered the presence of core subunits of type II secretion pathway (T2SS) in several unicellular eukaryotes (protists) belonging to jakobids, hetereloboseans and malawimonads. We show that the proteins carry mitochondrial signal sequences and are localized in the protist mitochondria.The mitochondria of Nagleria gruberi were isolated by differential centrifugation and analysed by mass spectrometry. The data analyses revealed about 1000 proteins enriched in the mitochondrial fraction including the components of T2SS. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:22:08.662.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Karel Harant |
SpeciesList | scientific name: Naegleria sp. NGES2; NCBI TaxID: 1437330; |
ModificationList | methylthiolated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-09-18 02:19:36 | ID requested | |
1 | 2021-05-19 07:37:43 | announced | |
⏵ 2 | 2024-10-22 05:22:09 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: evolution,mitochondria, protist, type two secretion |
Contact List
Mgr. Pavel Doležal, Ph.D. |
contact affiliation | „BIOCEV – Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University“ (CZ.1.05/1.1.00/02.0109) |
contact email | pavel.dolezal@natur.cuni.cz |
lab head | |
Karel Harant |
contact affiliation | Charles University |
contact email | harant@natur.cuni.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007764
- Label: PRIDE project
- Name: Proteomic analysis of Naegleria gruberi mitochondria