PXD007758

PXD007758 is an original dataset announced via ProteomeXchange.

Title	Vitamin C alters the amount of specific endoplasmic reticulum associated proteins involved in lipid metabolism in the liver of mice synthesizing a nonfunctional WRN mutant protein
Description	Werner syndrome (WS) is a premature aging disorder caused by mutations in a DNA helicase/exonuclease. Mice lacking the helicase domain of the Wrn protein orthologue exhibit transcriptomic and metabolic alterations, some of which are reversed by vitamin C. Recent studies on the liver of these animals indicated that the Wrn mutant protein is associated with the endoplasmic reticulum (ER) resulting in an ER stress response. In this study, we identified liver proteins that exhibit actual level differences in the ER fraction between wild type and Wrn mutant mice using quantitative proteomic profiling with label-free Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). Multiple Reaction Monitoring (MRM) and immunoblotting were performed to validate findings in a secondary independent cohort of wild type and Wrn mutant mice in the presence or absence of vitamin C in drinking water. The list of identified proteins showing significant altered expression levels was compared to transcriptomic results that were obtained in previous studies to assess the extent of correlation between the mRNA levels and their corresponding encoded protein levels in the liver of our different mouse cohorts.
HostingRepository	PRIDE
AnnounceDate	2018-03-02
AnnouncementXML	Submission_2018-03-02_08:17:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michel Lebel
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-18 01:54:57	ID requested
⏵ 1	2018-03-02 08:17:11	announced

Aumailley L, Roux-Dalvai F, Kelly I, Droit A, Lebel M, Vitamin C alters the amount of specific endoplasmic reticulum associated proteins involved in lipid metabolism in the liver of mice synthesizing a nonfunctional Werner syndrome (Wrn) mutant protein. PLoS One, 13(3):e0193170(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Werner syndrome, mouse model, Vitamin C, liver proteome, endoplasmic reticulum, LC-MSMS shotgun, Label Free quantification

Michel Lebel
contact affiliation	Centre de recherche du CHU de Québec, Faculty of Medicine, Université Laval, Quebec City Québec, Canada
contact email	michel.lebel@crchudequebec.ulaval.ca
lab head
Michel Lebel
contact affiliation	Centre Hospitalier de l’Université Laval (CHUL)
contact email	michel.lebel@crchudequebec.ulaval.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007758
     1. Label: PRIDE project
     2. Name: Vitamin C alters the amount of specific endoplasmic reticulum associated proteins involved in lipid metabolism in the liver of mice synthesizing a nonfunctional WRN mutant protein