PXD007756

PXD007756 is an original dataset announced via ProteomeXchange.

Title	The peptide repertoire of HLA-B27 includes ligands with lysine at P2 anchor position
Description	The HLA-B*27 peptidome has drawn significant attention due to the genetic association between some of the HLA-B*27 alleles and the inflammatory rheumatic disease ankylosing spondylitis (AS). The role of HLA-B*27 in this condition is not known yet. This study aims to expand the known limits of the HLA-B*27 peptidome in order to facilitate selection and testing of new peptides, possibly implicated with the disease. The HLA peptidomes of HeLa and C1R cell lines stably transfected with the AS-associated HLA-B*27:05 allele, the non-associated HLA-B*27:09 allele, or their mutants with Cysteine 67 replaced by Serine (C67S), were analyzed on a very large scale. In addition, the peptidomes of HLA-B*27:05 and HLA-B*27:05-C67S were analyzed from transgenic rats’ spleens. The results indicate that a fraction of HLA-B*27 peptides contain lysine at their second position (P2), in addition to the more commonly found peptides with arginine, or the less common glutamine located at this anchor position. Furthermore, the C67S mutation increases the percentages of peptides with glutamine or lysine at their P2 position, in both HLA-B*27:05 and HLA-B*27:09. Therefore, peptides with P2 lysine should be considered as valid ligands of HLA-B*27 molecules and taken into account while looking for candidate for arithrogenic peptides.
HostingRepository	PRIDE
AnnounceDate	2018-04-10
AnnouncementXML	Submission_2018-04-10_06:12:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dganit Melamed Kadosh
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-18 01:46:11	ID requested
⏵ 1	2018-04-10 06:12:04	announced

Yair-Sabag S, Tedeschi V, Vitulano C, Barnea E, Glaser F, Melamed Kadosh D, Taurog JD, Fiorillo MT, Sorrentino R, Admon A, The Peptide Repertoire of HLA-B27 may include Ligands with Lysine at P2 Anchor Position. Proteomics, 18(9):e1700249(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Human Leukocytes Antigen, HLA-B*27, Peptidome, Immunopeptidome, Ankylosing Spondylitis, Transgenic rats

Arie Admon
contact affiliation	Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel.
contact email	admon@technion.ac.il
lab head
Dganit Melamed Kadosh
contact affiliation	Technion
contact email	sdganit@tx.technion.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007756
     1. Label: PRIDE project
     2. Name: The peptide repertoire of HLA-B27 includes ligands with lysine at P2 anchor position