PXD007753

PXD007753 is an original dataset announced via ProteomeXchange.

Title	Trapping carbon dioxide on Arabidopsis proteome
Description	Carbon dioxide is vital to the chemistry of life processes including including metabolism, cellular homeostasis, and pathogenesis. CO2 forms carbamates on the neutral N-terminal a-amino- and lysine e-amino-groups that regulate the activities of ribulose 1,5-bisphosphate carboxylase/oxygenase and haemoglobin, however, very few protein other carbamates are known. Tools for the systematic identification of protein carbamylation sites have not been developed owing to the reversibility of carbamate formation, and in consequence carbamylation is typically overlooked. Here we demonstrate methods to identify protein carbamates using triethyloxonium ions to covalently trap CO2 on proteins for proteomic analysis. Our method delivers evidence to support the hypothesis that carbamylation is widespread in biology, and understanding its role should significantly advance our understanding of cellular CO2 interactions.
HostingRepository	PRIDE
AnnounceDate	2018-10-23
AnnouncementXML	Submission_2018-10-23_14:54:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Victoria Linthwaite
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	carbamoylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-18 01:31:32	ID requested
⏵ 1	2018-10-23 14:54:30	announced

Linthwaite VL, Janus JM, Brown AP, Wong-Pascua D, O'Donoghue AC, Porter A, Treumann A, Hodgson DRW, Cann MJ, The identification of carbon dioxide mediated protein post-translational modifications. Nat Commun, 9(1):3092(2018) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis, carbamate, carbon dioxide, trapping

Martin Cann
contact affiliation	Associate Professor in the Department of Biosciences Durham University UK
contact email	m.j.cann@durham.ac.uk
lab head
Victoria Linthwaite
contact affiliation	Durham University
contact email	v.l.linthwaite@durham.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007753

PRIDE project URI

• PRIDE
  1. PXD007753
     1. Label: PRIDE project
     2. Name: Trapping carbon dioxide on Arabidopsis proteome