PXD007748

PXD007748 is an original dataset announced via ProteomeXchange.

Title	Populus Drought Stress
Description	We sought to generate a comprehensive proteomic profile for a Populus plant that captures protein abundance behavioral differences in mature leaves under various water deficit conditions. The sudden infliction of severe drought is unlikely to reflect what naturally happens to soil-grown plants. Therefore, rather than exposing plants to severe dehydration via the application of concentrated osmotica (e.g., polyethylene glycol or mannitol), we aimed to monitor plants exposed to prolonged drought as well as plants frequently experiencing cyclic drought. For the progressive drought experiment, herein referred to as acute drought, water will be withheld from the plants for a certain period of time until symptoms of wilting are observed. From this drought treatment, protein abundance changes that occur because of declining water potentials that consequently lead to worsening plant health will provide new insights to biological pathways in response to severe drought conditions. On the other hand, as a consequence of simulating a more natural condition, the cyclic drought treatment will provide novel insights into drought recovery and coping mechanisms. As first noted in a meta-analysis of microarray experiments comparing different water deficit-related treatments3, one consequence of analyzing multiple treatment conditions is that we expect only a few differentially abundant proteins to be common to both treatments. Therefore, we implemented a bioinformatic framework to provide an overall integrative picture of the conserved drought markers but also meaningful divergences in functional behavioral responses between the two experiments.
HostingRepository	PRIDE
AnnounceDate	2017-11-16
AnnouncementXML	Submission_2018-02-23_06:11:17.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007748
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paul Abraham
SpeciesList	scientific name: Populus deltoides (Eastern poplar) (Eastern cottonwood); NCBI TaxID: 3696;
ModificationList	Oxidation; Carbamyl; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-17 23:49:45	ID requested
1	2017-11-16 03:44:13	announced
2	2018-02-22 01:58:33	announced	Updated publication reference for PubMed record(s): 29447168.
3	2018-02-23 00:34:35	announced	Updated publication reference for PubMed record(s): 29447168.
4	2018-02-23 04:38:35	announced	Updated publication reference for PubMed record(s): 29447168.
⏵ 5	2018-02-23 06:11:19	announced	Updated publication reference for PubMed record(s): 29447168.

Abraham PE, Garcia BJ, Gunter LE, Jawdy SS, Engle N, Yang X, Jacobson DA, Hettich RL, Tuskan GA, Tschaplinski TJ, Quantitative proteome profile of water deficit stress responses in eastern cottonwood (Populus deltoides) leaves. PLoS One, 13(2):e0190019(2018) [pubmed]

curator keyword: Biological

submitter keyword: LC-MS/MS, plant, drought, Populus

Paul E. Abraham
contact affiliation	Chemical Sciences Division at Oak Ridge National Laboratory, Oak Ridge, TN 37831
contact email	abrahampe@ornl.gov
lab head
Paul Abraham
contact affiliation	Oak Ridge National Laboratory
contact email	abrahampe@ornl.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007748

PRIDE project URI

• PRIDE
  1. PXD007748
     1. Label: PRIDE project
     2. Name: Populus Drought Stress