PXD007745 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative Proteomics Reveals Specialized Wiring of Signaling Pathways that Protects Human Regulatory T Cell Identity - 2 populations in vitro |
Description | Regulatory CD4+ T cells (Tregs) are functionally distinct from conventional CD4+ T cells (Tconvs). To understand Treg identity, we compared by proteomics and transcriptomics human naïve (n) and effector (e)Tregs, Tconvs and transitional FOXP3+ cells. Only 12% of 422 differentially expressed proteins was identified as such at the mRNA level, demonstrating the importance of direct proteome measurement. Fifty-one proteins discriminated Tregs from Tconvs. This common Treg protein signature indicates altered signaling by TCR-, TNF receptor-, NFB-, PI3 kinase/mTOR-, NFAT- and STAT pathways, and unique cell biological and metabolic features. Another protein signature uniquely identified eTregs and revealed active cell division, apoptosis sensitivity and suppression of NFB- and STAT signaling. eTreg fate appears consolidated by FOXP3 outnumbering its partner transcription factors. These features explain why eTregs cannot produce inflammatory cytokines, whereas transitional FOXP3+ cells can. Collectively, our data reveal that Treg identity is defined and protected by uniquely “wired” signaling pathways. |
HostingRepository | PRIDE |
AnnounceDate | 2018-05-18 |
AnnouncementXML | Submission_2018-05-18_06:24:46.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Maartje van den Biggelaar |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion ETD |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-09-15 01:10:57 | ID requested | |
⏵ 1 | 2018-05-18 06:24:47 | announced | |
Publication List
Cuadrado E, van den Biggelaar M, de Kivit S, Chen YY, Slot M, Doubal I, Meijer A, van Lier RAW, Borst J, Amsen D, Proteomic Analyses of Human Regulatory T Cells Reveal Adaptations in Signaling Pathways that Protect Cellular Identity. Immunity, 48(5):1046-1059.e6(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Human, Blood, CD4+ T lymphocytes |
Contact List
Maartje van den Biggelaar |
contact affiliation | Sanquin Research, department of Plasma Proteins, Plesmanlaan 125, 1066 CX Amsterdam, the Netherlands |
contact email | m.vandenbiggelaar@sanquin.nl |
lab head | |
Maartje van den Biggelaar |
contact affiliation | Sanquin Research |
contact email | m.vandenbiggelaar@sanquin.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007745
- Label: PRIDE project
- Name: Quantitative Proteomics Reveals Specialized Wiring of Signaling Pathways that Protects Human Regulatory T Cell Identity - 2 populations in vitro