PXD007734

PXD007734 is an original dataset announced via ProteomeXchange.

Title	Protein analysis from mandibular glands of termites
Description	a. After collection, live termites were brought to geneOmbio Technologies Central Processing Laboratory under controlled room temperature (25.9C, 72% RH) until dissection was made. The termites selected for analysis were collected from Pune region (18°31′13″N 73°51′24″E) from the state of Maharashtra, India. These were identified as Hypotermes xenotermitis based on mitochondrial COI gene sequencing. b. Based on the morphological features the insects were segregated into two groups (a) workers (b) soldiers c. Ten termites from each group were anaesthetized on ice and immediately dissected for isolation of mandibular glands (circled) using sterile scalpel, blade and petri plate. d. The glands were transferred in two milliliter sterile Tris HCl buffer (pH 7.0) containing 0.1 M NaCl for SDS-PAGE analysis. Duplicate samples were collected for each workers and soldiers. Hence total four protein samples were prepared. e. The sample was homogenized with a teflon pestle homogenizer and stored at -20 C till use.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:40:25.058.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yashwant Chavan
SpeciesList	scientific name: Hypotermes; NCBI TaxID: 73854;
ModificationList	No PTMs are included in the dataset
Instrument	6410 Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-13 03:17:40	ID requested
1	2019-04-25 02:20:54	announced
⏵ 2	2024-10-22 04:40:25	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: mandibular gland, proteome,zootermosis

Dr Bhaskar Jadhav
contact affiliation	Head, Department of Life Sciences, Mumbai University
contact email	drbljadhav@gmail.com
lab head
Yashwant Chavan
contact affiliation	geneOmbio Technologies Private Limited
contact email	visualyash10@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD007734

PRIDE project URI

• PRIDE
  1. PXD007734
     1. Label: PRIDE project
     2. Name: Protein analysis from mandibular glands of termites