PXD007732

PXD007732 is an original dataset announced via ProteomeXchange.

Title	The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of SMC4 involved in replicative senescence
Description	In this study, a label-free quantitative proteomic approach was employed to analyze the serial passaged human skin fibroblast （CCD-1079Sk） cells, within 3305 proteins quantified. Of which, 372 proteins were significantly changed in early passage(P6), middle passage (P12) and later passage (P21), with a time-dependent decrease or increase tendency. Then, of which, SMC4 was selected for further biological validation. The results confirmed that the expression of SMC4 was significantly down-regulated in a time-dependent manner in the subculture of human skin fibroblasts (HSFb) with Western Blot experiment.
HostingRepository	PRIDE
AnnounceDate	2018-10-22
AnnouncementXML	Submission_2018-10-22_01:09:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MENG QIAN
SpeciesList	scientific name: Acyrthosiphon pisum (Pea aphid); NCBI TaxID: 7029; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-13 01:30:57	ID requested
⏵ 1	2018-10-22 01:09:34	announced

Meng Q, Gao J, Zhu H, He H, Lu Z, Hong M, Zhou H, The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of the chromosome condensin complex proteins involved in replicative senescence. Biochem Biophys Res Commun, 505(4):1112-1120(2018) [pubmed]

curator keyword: Biological

submitter keyword: proteomic study

human skin fibroblasts

serially passaged

replicative senescence

SMC4

zhou hu
contact affiliation	SIMM
contact email	zhouhu@simm.ac.cn
lab head
MENG QIAN
contact affiliation	SIMM
contact email	qianmeng@simm.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007732
     1. Label: PRIDE project
     2. Name: The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of SMC4 involved in replicative senescence