PXD007730

PXD007730 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	interferon-induced global cellular SUMOylation Changes
Description	This proteomics dataset was generated to study the changes in SUMO site abundances between control cells and Interferon-alpha treated cells using SILAC. Cells were first fractionated into the cytoplasmic and nuclear fractions. SUMOylated proteins were then purified from these extracts by Ni-NTA via the 6xHIS tag on the SUMO3 construct. The proteins were then digested with trypsin and the peptides desalted on HLB cartridges. The SUMOylated peptides, which harbor an NQTGG remnant after tryptic digestion, were enriched with our anti-NQTGG antibody. The resulting peptides were injected on the orbitrap fusion instrument. The .raw files were analyzed with maxqunt.
HostingRepository	MassIVE
AnnounceDate	2018-04-11
AnnouncementXML	Submission_2018-04-11_08:53:11.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francis McManus
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	unknown modification: [K] NQTGG SUMO3 remnant
Instrument	Orbitrap Fusion ETD

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-12 10:55:33	ID requested
⏵ 1	2018-04-11 08:53:12	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: SUMO, Interferon alpha, PML, UBC9

submitter keyword: SUMO, Interferon alpha, PML, UBC9

Contact List

Mounira K. Chelbi-Alix
contact affiliation	INSERM UMR-S1124
contact email	mounira.chelbi-alix@parisdescartes.fr
lab head
Pierre Thibault
contact affiliation	University of Montreal
contact email	pierre.thibault@umontreal.ca
lab head
Francis McManus
contact affiliation	University of Montreal
contact email	francis.mcmanus@umontreal.ca
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000081525

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000081525