PXD007726 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A novel USP9X substrate TTK contributes to tumorigenesis in non-small cell lung cancer |
Description | The X-linked deubiquitinase, USP9X, is implicated in multiple cancers by targeting various substrates. Increased expression of USP9X is observed in non-small cell lung cancer (NSCLC) and is correlated with poor prognosis. However, the molecular mechanism for USP9X regulating tumor cell survival and tumorigenesis in NSCLC is less defined. In this study, chemical labeling quantitative proteomic screening was applied to analyze A549 cells with or without USP9X RNA interference, and the resulting data suggested that TTK is a potential substrate of USP9X. Further experimental evidences confirmed that USP9X stabilized TTK via direct interaction and deubiquitination of TTK. Moreover, knockdown of USP9X or TTK inhibited cell proliferation, migration and tumorigenesis, and the immunohistochemical analysis of clinical NSCLC samples showed that the protein expression levels of USP9X and TTK were significantly elevated and positively correlated in tumor tissues. In summary, our data demonstrated that the USP9X-TTK axis may play a critical role in NSCLC, and could be considered as the potential therapeutic target. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:40:47.131.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jing Gao |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-09-12 02:24:50 | ID requested | |
1 | 2018-05-07 00:51:09 | announced | |
⏵ 2 | 2024-10-22 04:40:53 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.7150/thno.22901; |
Chen X, Yu C, Gao J, Zhu H, Cui B, Zhang T, Zhou Y, Liu Q, He H, Xiao R, Huang R, Xie H, Gao D, Zhou H, A novel USP9X substrate TTK contributes to tumorigenesis in non-small-cell lung cancer. Theranostics, 8(9):2348-2360(2018) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: non-small cell lung cancer (NSCLC) |
quantitative proteomics |
deubiquitinase |
USP9X |
TTK |
Contact List
Zhou Hu |
contact affiliation | Shanghai Institute of Materia Medica, Chinese Academy of Sciences |
contact email | zhouhu@simm.ac.cn |
lab head | |
Jing Gao |
contact affiliation | Shanghai Institute of Materia Medica, Chinese Academy of Science |
contact email | jinggao@simm.ac.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007726
- Label: PRIDE project
- Name: A novel USP9X substrate TTK contributes to tumorigenesis in non-small cell lung cancer