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PXD007726

PXD007726 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA novel USP9X substrate TTK contributes to tumorigenesis in non-small cell lung cancer
DescriptionThe X-linked deubiquitinase, USP9X, is implicated in multiple cancers by targeting various substrates. Increased expression of USP9X is observed in non-small cell lung cancer (NSCLC) and is correlated with poor prognosis. However, the molecular mechanism for USP9X regulating tumor cell survival and tumorigenesis in NSCLC is less defined. In this study, chemical labeling quantitative proteomic screening was applied to analyze A549 cells with or without USP9X RNA interference, and the resulting data suggested that TTK is a potential substrate of USP9X. Further experimental evidences confirmed that USP9X stabilized TTK via direct interaction and deubiquitination of TTK. Moreover, knockdown of USP9X or TTK inhibited cell proliferation, migration and tumorigenesis, and the immunohistochemical analysis of clinical NSCLC samples showed that the protein expression levels of USP9X and TTK were significantly elevated and positively correlated in tumor tissues. In summary, our data demonstrated that the USP9X-TTK axis may play a critical role in NSCLC, and could be considered as the potential therapeutic target.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:40:47.131.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJing Gao
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-09-12 02:24:50ID requested
12018-05-07 00:51:09announced
22024-10-22 04:40:53announced2024-10-22: Updated project metadata.
Publication List
10.7150/thno.22901;
Chen X, Yu C, Gao J, Zhu H, Cui B, Zhang T, Zhou Y, Liu Q, He H, Xiao R, Huang R, Xie H, Gao D, Zhou H, A novel USP9X substrate TTK contributes to tumorigenesis in non-small-cell lung cancer. Theranostics, 8(9):2348-2360(2018) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: non-small cell lung cancer (NSCLC)
quantitative proteomics
deubiquitinase
USP9X
TTK
Contact List
Zhou Hu
contact affiliationShanghai Institute of Materia Medica, Chinese Academy of Sciences
contact emailzhouhu@simm.ac.cn
lab head
Jing Gao
contact affiliationShanghai Institute of Materia Medica, Chinese Academy of Science
contact emailjinggao@simm.ac.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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