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PXD007725

PXD007725 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomic Analysis Reveals up-regulation of caveolin-1 in FOXP3-overexpressed human gastric cancer cells
DescriptionForkhead box protein 3 (FOXP3) is implicated in tumor progression and prognosis in various types of tumor cells. We have recently reported that FOXP3 inhibited proliferation of gastric cancer (GC) cells through activating the apoptotic signaling pathway. In this study, we found that over-expression of FOXP3 inhibited GC cell migration, invasion and proliferation. Then, the label-free quantitative proteomic approach was employed to further investigating the down-stream proteins regulated by FOXP3, resulting in a total of 3,978 proteins quantified, including 186 significantly changed proteins. Caveolin-1 (CAV1), as a main constituent protein of caveolae, was one of those changed proteins up-regulated in FOXP3-overexpressed GC cells, moreover, it was assigned as one of the node proteins in the protein-protein interaction network and the key protein involved in focal adhesion pathway by bioinformatics analysis. Further biological experiments confirmed that FOXP3 directly bound to the promoter regions of CAV1 to positively regulate CAV1 transcription in GC cells. In summary, our study suggested that FOXP3 can be considered as a tumor suppressor in GC via positively regulating CAV1 through transcriptional activation, and this FOXP3-CAV1 transcriptional regulation axis may play an important role in inhibiting invasion and metastasis of GC cells.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:40:27.007.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJing Gao
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-09-12 02:24:23ID requested
12017-11-03 03:22:18announced
22024-10-22 04:40:35announced2024-10-22: Updated project metadata.
Publication List
Pan D, Gao J, Zeng X, Ma G, Li N, Huang X, Du X, Miao Q, Lian J, Xu L, Zhou H, Chen S, Quantitative proteomic Analysis Reveals up-regulation of caveolin-1 in FOXP3-overexpressed human gastric cancer cells. Sci Rep, 7(1):14460(2017) [pubmed]
10.1038/s41598-017-14453-2;
Keyword List
curator keyword: Biomedical
submitter keyword: FOXP3
CAV1
gastric cancer
label-free proteomic analysis
Contact List
Zhou Hu
contact affiliationShanghai Institute of Materia Medica, Chinese Academy of Sciences
contact emailzhouhu@simm.ac.cn
lab head
Jing Gao
contact affiliationShanghai Institute of Materia Medica, Chinese Academy of Science
contact emailjinggao@simm.ac.cn
dataset submitter
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Dataset FTP location
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