PXD007721

PXD007721 is an original dataset announced via ProteomeXchange.

Title	Characterization of syntenin knockout exosomes from 4T1 murine breast cancer cells
Description	Tumor cells are known to induce tumor-supporting phenotypes in surrounding stroma cells which leads to the creation of a favourable tumor niche and sustained growth as well as progression of the tumor. One mechanism how tumor cells can modulate surrounding stroma cells is through the release of extracellular vesicles. These vesicles comprise large plasma membrane-derived microvesicles (diameter 100-1000 nm) as well as the better characterized smaller exosomes (diameter <100 nm) which are derived from intraluminal vesicles inside endosomal compartments. In spite of the identification of vesicle-mediated tumor-tumor and tumor-stroma intracellular communication loops, the tumor-supporting factors present on tumor vesicles as well as the factors that regulate their export are largely unknown. One attractive candidate protein which could be involved in controlling vesicular cargo selection is the adaptor scaffolding protein syntenin/SDCBP, which is known as a marker protein highly expressed on exosomes. Syntenin is characterized by two structural protein interaction modules, the PDZ domains, which can either bind to other PDZ domains or to the C-terminus of target proteins in a sequence-specific way, thereby enabling protein-protein interactions. PDZ proteins in general are known to be involved in the recruitment and assembly of specific protein complexes, especially at the cellular plasma membrane. Syntenin is a factor that has been shown to control not only the biogenesis of exosomes, but also the sorting of specific proteins to these vesicles. While it is highly expressed in human fetal tissues, its expression is significantly downregulated in adult tissues with the exception of the heart or placenta. However, high levels of syntenin seem to be restored in advanced human cancer of different entities and thereby suggest a role for syntenin in tumor progression. Previous results from the group of Pascale Zimmermann showed that syntenin is of particular importance for regulating the sorting of its (known) target proteins to exosomes which would allow their distribution to neighboring stroma cells. To analyze which tumor-supporting factors are present on breast cancer exosomes and which of them are regulated by syntenin, exosomes from 4T1 wildtype cells (syntenin-high) and from two clones of syntenin CRISPR/Cas9 syntenin knockout cells were isolated from cell culture supernatants by differential ultracentrifugation and then analyzed and compared by label-free mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_17:52:55.428.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luc Camoin
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-12 01:59:42	ID requested
⏵ 1	2025-08-24 17:52:56	announced

Irmer B, Angenendt A, Camoin L, Audebert S, Geyer C, Gerwing M, Spiessbach H, Hebel M, Baudelet É, Wlochowitz D, Hansen U, Bleckmann A, Zimmermann P, Menck K, Syntenin Controls Extracellular Vesicle-Induced Tumour Migration by Regulating the Expression of Adhesion Proteins on Small Extracellular Vesicles. J Extracell Vesicles, 14(8):e70133(2025) [pubmed]

10.1002/jev2.70133;

curator keyword: Biological, Biomedical

submitter keyword: breast cancer, syntenin, CRISPR/Cas9,Exosomes

Luc Camoin
contact affiliation	Marseille proteomique, Inserm U1068, CNRS UMR 7258, Aix marseille University UM105
contact email	luc.camoin@inserm.fr
lab head
Luc Camoin
contact affiliation	Life Sciences
contact email	luc.camoin@inserm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007721
     1. Label: PRIDE project
     2. Name: Characterization of syntenin knockout exosomes from 4T1 murine breast cancer cells