PXD007719

PXD007719 is an original dataset announced via ProteomeXchange.

Title	The role of the sRNA GcvB in Pasteurella multocida protein production
Description	In this study, transcriptomic analysis of the P. multocida strain VP161 revealed a putative sRNA with high identity to GcvB from Escherichia coli and Salmonella enterica serovar Typhimurium. High-throughput quantitative liquid proteomics was used to compare the proteomes of the P. multocida VP161 wild-type strain, a gcvB mutant and a GcvB overexpression strain. These analyses identified 47 proteins that displayed significant differential production after inactivation of gcvB, 37 of which showed increased production. Thus, GcvB predominantly acts to negatively regulate protein production in P. multocida. Of the 37 proteins that were repressed by GcvB, 27 were predicted to be involved in amino acid biosynthesis or transport. Bioinformatic analyses of putative P. multocida GcvB target mRNAs identified a strongly conserved 10 nucleotide consensus sequence, 5’‑AACACAACAT-3’, with the central eight nucleotides identical to the seed binding region present within GcvB mRNA targets in E. coli and S. Typhimurium.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:40:08.534.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ralf Schittenhelm
SpeciesList	scientific name: Pasteurella multocida; NCBI TaxID: 747;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-12 01:45:15	ID requested
1	2019-11-12 09:44:22	announced
⏵ 2	2024-10-22 04:40:16	announced	2024-10-22: Updated project metadata.

Gulliver EL, Wright A, Lucas DD, M, é, groz M, Kleifeld O, Schittenhelm RB, Powell DR, Seemann T, Bulitta JB, Harper M, Boyce JD, and identification of the GcvB seed binding region. RNA, 24(5):704-720(2018) [pubmed]

10.1261/rna.063248.117;

curator keyword: Biological

submitter keyword: LC-MS/MS, Pasteurella multocida, GcvB

John D. Boyce
contact affiliation	Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, Victoria, Australia
contact email	john.boyce@monash.edu
lab head
Ralf Schittenhelm
contact affiliation	Monash University
contact email	ralf.schittenhelm@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD007719

PRIDE project URI

• PRIDE
  1. PXD007719
     1. Label: PRIDE project
     2. Name: The role of the sRNA GcvB in Pasteurella multocida protein production