PXD007713

PXD007713 is an original dataset announced via ProteomeXchange.

Title	Molecular Profile of Priapism Associated with Low Nitric Oxide Bioavailability
Description	Priapism is a disorder in which prolonged penile erection persists uncontrollably, potentially leading to tissue damage. Priapism commonly afflicts patient populations with severely low nitric oxide (NO) bioavailability. As NO is a primary mediator of erection, the molecular mechanisms involved in priapism pathophysiology associated with low NO bioavailability are not well understood. The objective of this study was to identify novel molecular targets and signaling pathways in a mouse model of low NO bioavailability. Neuronal + endothelial NO synthase double knockout mice (NOS1/3-/-) were used as a model of low NO bioavailability. Priapic activity was demonstrated in the NOS1/3-/- mice relative to wild type (WT) mice by measurement of prolonged erections following cessation of electrical stimulation of the cavernous nerve. Penile tissue was processed and analyzed by reversed-phase liquid chromatography tandem mass spectrometry. 1353 total proteins were identified and quantified by spectral counting, 42 of which were downregulated and 113 of which were upregulated in NOS1/3-/- vs. WT (P<0.05). Ingenuity Pathway Analysis of differentially expressed proteins revealed increased protein kinase A signaling, G-protein coupled receptor signaling, and decreased adenosine catabolism in NOS1/3-/- penis all represent potential compensatory mechanisms that may play a role in priapism secondary to low NO bioavailability.
HostingRepository	PRIDE
AnnounceDate	2018-03-13
AnnouncementXML	Submission_2018-03-13_03:43:30.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007713
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Vidya Venkatraman
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-11 03:31:53	ID requested
⏵ 1	2018-03-13 03:43:31	announced

La Favor JD, Fu Z, Venkatraman V, Bivalacqua TJ, Van Eyk JE, Burnett AL, Molecular Profile of Priapism Associated with Low Nitric Oxide Bioavailability. J Proteome Res, 17(3):1031-1040(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Priapism, nitric oxide, erectile, mass spectrometry, PKA, GPCR, penis, proteomics

Dr. Jennifer Van Eyk
contact affiliation	Advanced Clinical Biosystems Research Institute, Department of Medicine and The Heart Institute, Cedars-Sinai Medical Center, Los Angeles, California 90048, United States
contact email	jennifer.vaneyk@cshs.org
lab head
Vidya Venkatraman
contact affiliation	Cedars-Sinai Medical Center
contact email	vidya.venkatraman@cshs.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007713
     1. Label: PRIDE project
     2. Name: Molecular Profile of Priapism Associated with Low Nitric Oxide Bioavailability