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PXD007713

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-03-13
  • AnnouncementXML: Submission_2018-03-13_03:43:30.xml
  • DigitalObjectIdentifier: http://dx.doi.org/10.6019/PXD007713
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Vidya Venkatraman
  • Title: Molecular Profile of Priapism Associated with Low Nitric Oxide Bioavailability
  • Description: Priapism is a disorder in which prolonged penile erection persists uncontrollably, potentially leading to tissue damage. Priapism commonly afflicts patient populations with severely low nitric oxide (NO) bioavailability. As NO is a primary mediator of erection, the molecular mechanisms involved in priapism pathophysiology associated with low NO bioavailability are not well understood. The objective of this study was to identify novel molecular targets and signaling pathways in a mouse model of low NO bioavailability. Neuronal + endothelial NO synthase double knockout mice (NOS1/3-/-) were used as a model of low NO bioavailability. Priapic activity was demonstrated in the NOS1/3-/- mice relative to wild type (WT) mice by measurement of prolonged erections following cessation of electrical stimulation of the cavernous nerve. Penile tissue was processed and analyzed by reversed-phase liquid chromatography tandem mass spectrometry. 1353 total proteins were identified and quantified by spectral counting, 42 of which were downregulated and 113 of which were upregulated in NOS1/3-/- vs. WT (P<0.05). Ingenuity Pathway Analysis of differentially expressed proteins revealed increased protein kinase A signaling, G-protein coupled receptor signaling, and decreased adenosine catabolism in NOS1/3-/- penis all represent potential compensatory mechanisms that may play a role in priapism secondary to low NO bioavailability.
  • SpeciesList: scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
  • ModificationList: Oxidation; Carbamidomethyl
  • Instrument: LTQ Orbitrap Elite

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-09-11 03:31:53ID requested
12018-03-13 03:43:31announced

Publication List

  1. La Favor JD, Fu Z, Venkatraman V, Bivalacqua TJ, Van Eyk JE, Burnett AL, Molecular Profile of Priapism Associated with Low Nitric Oxide Bioavailability. J Proteome Res, 17(3):1031-1040(2018) [pubmed]

Keyword List

  1. curator keyword: Biomedical
  2. submitter keyword: Priapism, nitric oxide, erectile, mass spectrometry, PKA, GPCR, penis, proteomics

Contact List

    Dr. Jennifer Van Eyk
    • contact affiliation: Advanced Clinical Biosystems Research Institute, Department of Medicine and The Heart Institute, Cedars-Sinai Medical Center, Los Angeles, California 90048, United States
    • contact email: jennifer.vaneyk@cshs.org
    • lab head:
    Vidya Venkatraman
    • contact affiliation: Cedars-Sinai Medical Center
    • contact email: vidya.venkatraman@cshs.org
    • dataset submitter:

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