PXD007712

PXD007712 is an original dataset announced via ProteomeXchange.

Title	Tick host range adaptation: changes in protein profiles in Ixodes scapularis and Amblyomma americanum saliva during stimulation to feed on different hosts
Description	Understanding the molecular basis of how the tick adapts to feed on different animal hosts is central to understanding tick and tick-borne disease (TBD) epidemiology. Tick adaptation to feed on vertebrate hosts is regulated by tick secretion of multiple tick saliva proteins (TSPs) and other molecules that regulate tick feeding. This study was initiated to determine if ticks such as Ixodes scapularis and Amblyomma americanum that are adapted to feed on multiple hosts utilized the same sets of proteins to accomplish feeding on all hosts. Our data suggest that ticks of the same species differentially express proteins when feeding on diffent hosts. SDS-PAGE and silver staining analysis revealed unique protein eletrophoretic profile in saliva of Ixodes scapularis and Amblyomma americanum that were stimulated to start feeding on different hosts: rabbits, humans, and dogs. LC-MS/MS sequencing and pairwise analysis of proteins in saliva of I. scapularis and A. americanum ticks that were non-stimulated and those that were stimulated to feed on rabbits, dogs, or humans identified TSPs that were unique to each treatment and those that were common. Overal, we identified a total of 276 and 340 non-redundant I. scapularis and A. americanum TSPs, which we have classified into 28 functional classes that include secreted conserved proteins (unknown functions), proteinase inhibitors, lipocalins, extracellular matrix/cell adhesion, heme/iron metabolism, signal transduction and immunity-related proteins being the most predominant in saliva of unfed ticks. With exception of Rhipicephalus microplus, anti-tick vaccine research relies on feeding lab animals. Data here suggest that lab animal data could result in prioritizing irrelevant targets as some tick genes are unique to ticks fed on lab animals. This study provides the platform that could be utilized to identify relevant target anti-tick vaccine antigens, and will facilitate early stage tick feeding research.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:33:58.371.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Albert Mulenga
SpeciesList	scientific name: Amblyomma americanum; NCBI TaxID: 6943; scientific name: Ixodes scapularis (Black-legged tick) (Deer tick); NCBI TaxID: 6945;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-11 02:23:33	ID requested
1	2022-02-28 02:35:11	announced
⏵ 2	2024-10-22 05:33:59	announced	2024-10-22: Updated project metadata.

Tirloni L, Kim TK, Pinto AFM, Yates JR, da Silva Vaz I, Mulenga A, Saliva Stimulated to Feed on Different Hosts. Front Cell Infect Microbiol, 7():517(2017) [pubmed]

10.3389/fcimb.2017.00517;

curator keyword: Biological, Biomedical

submitter keyword: tick-host relationship, saliva,tick, proteomic, host adaptation

Albert Mulenga
contact affiliation	Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX, USA
contact email	amulenga@cvm.tamu.edu
lab head
Albert Mulenga
contact affiliation	Texas A&M University
contact email	mulengalab06@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007712
     1. Label: PRIDE project
     2. Name: Tick host range adaptation: changes in protein profiles in Ixodes scapularis and Amblyomma americanum saliva during stimulation to feed on different hosts