PXD007703

PXD007703 is an original dataset announced via ProteomeXchange.

Title	Cell type-specific metabolic labelling of nascent proteomes in vivo
Description	Changes in protein expression drive both the acute responses and long-lasting adaptation of cells to internal and external stimuli. While advances in labelling strategies have made it possible to examine general proteome dynamics, it is still not possible to access the proteomes of specific cell types in vivo in vertebrates. Here we describe a transgenic mouse line where Cre-recombinase-induced expression of a mutant methionyl-tRNA synthetase (L274G) enables the cell type-specific labelling of nascent proteins with a non-canonical amino-acid and click chemistry. Using immunoblotting, imaging and mass spectrometry, we demonstrate the metabolic labelling of proteins in genetically-targeted neurons in brain slices and in vivo. Moreover, we discover over 200 proteins that are regulated in hippocampal excitatory neurons by exposing mice to an enriched environment. As such, this approach opens new avenues for the isolation, analysis and quantitative comparison of cell type-specific proteomes and their dynamics in healthy and diseased tissues.
HostingRepository	PRIDE
AnnounceDate	2017-11-10
AnnouncementXML	Submission_2017-11-10_10:18:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Langer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive Plus; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-10 23:41:00	ID requested
⏵ 1	2017-11-10 10:18:45	announced

Alvarez-Castelao B, Schanzenb, ä, cher CT, Hanus C, Glock C, Tom Dieck S, D, ö, rrbaum AR, Bartnik I, Nassim-Assir B, Ciirdaeva E, Mueller A, Dieterich DC, Tirrell DA, Langer JD, Schuman EM, Cell-type-specific metabolic labeling of nascent proteomes in vivo. Nat Biotechnol, 35(12):1196-1201(2017) [pubmed]

curator keyword: Biological

submitter keyword: Metabolic labeling, nascent proteomes, Azidonorleucine, ANL, BONCAT, FUNCAT

Julian David Langer
contact affiliation	MPI of Biophysics and MPI for Brain Research
contact email	julian.langer@biophys.mpg.de
lab head
Julian Langer
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007703

PRIDE project URI

• PRIDE
  1. PXD007703
     1. Label: PRIDE project
     2. Name: Cell type-specific metabolic labelling of nascent proteomes in vivo