PXD007698

PXD007698 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of IL33-proximal proteins in squamous cell carcinoma cells
Description	Focal adhesion kinase (FAK) is required for the expression of pro-inflammatory genes that inhibit anti-tumour immunity. Here, we show a novel and crucial role for the dual-function cytokine IL33 in FAK-dependent immune evasion. Specifically, nuclear FAK is required for the expression of IL33, the chemokine Ccl5 and the soluble form of the IL33 receptor, sST2, which are required for evasion of CD8+ T-cell-mediated anti-tumour immunity. Mechanistically, nuclear IL33 associates with FAK and a network of chromatin modifiers and transcriptional regulators, including Taf9, WDR82 and BRD4, while sST2 likely negates effects of IL33 secreted into the tumour microenvironment by infiltrating host immune cells. Finally, protein interaction network analysis implies that nuclear FAK–IL33 complexes impact on transcription factors that regulate NFkB and chemokines like Ccl5 downstream. Our data therefore provide new mechanistic insight into how FAK controls the tumour immune environment via a FAK–IL33/sST2 pathway and demonstrate a novel role for nuclear IL33 downstream of FAK as a component of transcription regulatory complexes that critically modulate anti-tumour immunity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:40:13.733.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adam Byron
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-08 05:55:32	ID requested
1	2017-12-06 06:17:22	announced
2	2017-12-11 04:08:37	announced	Updated publication reference for PubMed record(s): 29208683.
⏵ 3	2024-10-22 04:40:14	announced	2024-10-22: Updated project metadata.

Serrels B, McGivern N, Canel M, Byron A, Johnson SC, McSorley HJ, Quinn N, Taggart D, Von Kreigsheim A, Anderton SM, Serrels A, Frame MC, IL-33 and ST2 mediate FAK-dependent antitumor immune evasion through transcriptional networks. Sci Signal, 10(508):(2017) [pubmed]

10.1126/scisignal.aan8355;

curator keyword: Biological, Biomedical

submitter keyword: FAK, transcription, proteomics, tumour growth, LC-MS, IL33, IL1RL1, SCC, BioID, ST2, chromatin

Margaret C Frame
contact affiliation	Cancer Research UK Edinburgh Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, United Kingdom
contact email	m.frame@ed.ac.uk
lab head
Adam Byron
contact affiliation	University of Manchester
contact email	adam.byron@igmm.ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD007698

PRIDE project URI

• PRIDE
  1. PXD007698
     1. Label: PRIDE project
     2. Name: Proteomic analysis of IL33-proximal proteins in squamous cell carcinoma cells