PXD007698 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of IL33-proximal proteins in squamous cell carcinoma cells |
Description | Focal adhesion kinase (FAK) is required for the expression of pro-inflammatory genes that inhibit anti-tumour immunity. Here, we show a novel and crucial role for the dual-function cytokine IL33 in FAK-dependent immune evasion. Specifically, nuclear FAK is required for the expression of IL33, the chemokine Ccl5 and the soluble form of the IL33 receptor, sST2, which are required for evasion of CD8+ T-cell-mediated anti-tumour immunity. Mechanistically, nuclear IL33 associates with FAK and a network of chromatin modifiers and transcriptional regulators, including Taf9, WDR82 and BRD4, while sST2 likely negates effects of IL33 secreted into the tumour microenvironment by infiltrating host immune cells. Finally, protein interaction network analysis implies that nuclear FAK–IL33 complexes impact on transcription factors that regulate NFkB and chemokines like Ccl5 downstream. Our data therefore provide new mechanistic insight into how FAK controls the tumour immune environment via a FAK–IL33/sST2 pathway and demonstrate a novel role for nuclear IL33 downstream of FAK as a component of transcription regulatory complexes that critically modulate anti-tumour immunity. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:40:13.733.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Adam Byron |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-09-08 05:55:32 | ID requested | |
1 | 2017-12-06 06:17:22 | announced | |
2 | 2017-12-11 04:08:37 | announced | Updated publication reference for PubMed record(s): 29208683. |
⏵ 3 | 2024-10-22 04:40:14 | announced | 2024-10-22: Updated project metadata. |
Publication List
Serrels B, McGivern N, Canel M, Byron A, Johnson SC, McSorley HJ, Quinn N, Taggart D, Von Kreigsheim A, Anderton SM, Serrels A, Frame MC, IL-33 and ST2 mediate FAK-dependent antitumor immune evasion through transcriptional networks. Sci Signal, 10(508):(2017) [pubmed] |
10.1126/scisignal.aan8355; |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: FAK, transcription, proteomics, tumour growth, LC-MS, IL33, IL1RL1, SCC, BioID, ST2, chromatin |
Contact List
Margaret C Frame |
contact affiliation | Cancer Research UK Edinburgh Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, United Kingdom |
contact email | m.frame@ed.ac.uk |
lab head | |
Adam Byron |
contact affiliation | University of Manchester |
contact email | adam.byron@igmm.ed.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007698
- Label: PRIDE project
- Name: Proteomic analysis of IL33-proximal proteins in squamous cell carcinoma cells