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PXD007695

PXD007695 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLipid droplet-targeted APEX proteome
DescriptionLipid droplet (LD) function is regulated by a complement of integral and peripheral proteins that associate with the LD phospholipid monolayer. Defining the composition of the LD proteome has remained a challenge due to the presence of contaminating proteins in LD-enriched buoyant fractions. To overcome this limitation, we developed a proximity labeling strategy that exploits LD-targeted APEX2 to biotinylate LD proteins in living cells. Application of this approach to U2OS and Huh7 cells identified the vast majority of previously validated LD proteins, excluded common contaminating proteins, and revealed new LD proteins.
HostingRepositoryPRIDE
AnnounceDate2017-12-22
AnnouncementXMLSubmission_2018-05-23_07:23:13.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJames Olzmann
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListbiotinylated residue
Instrument6410 Triple Quadrupole LC/MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-09-08 05:49:56ID requested
12017-12-22 00:14:17announced
22018-05-23 07:23:14announcedUpdated publication reference for PubMed record(s): 29275994.
Publication List
Bersuker K, Peterson CWH, To M, Sahl SJ, Savikhin V, Grossman EA, Nomura DK, Olzmann JA, A Proximity Labeling Strategy Provides Insights into the Composition and Dynamics of Lipid Droplet Proteomes. Dev Cell, 44(1):97-112.e7(2018) [pubmed]
Keyword List
curator keyword: Technical, Biological
submitter keyword: Lipid droplet, APEX, ERAD
Contact List
James Olzmann
contact affiliationUniversity of California, Berkeley
contact emailolzmann@berkeley.edu
lab head
James Olzmann
contact affiliationUniversity of California, Berkeley
contact emailjolzmann@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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