PXD007695 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Lipid droplet-targeted APEX proteome |
Description | Lipid droplet (LD) function is regulated by a complement of integral and peripheral proteins that associate with the LD phospholipid monolayer. Defining the composition of the LD proteome has remained a challenge due to the presence of contaminating proteins in LD-enriched buoyant fractions. To overcome this limitation, we developed a proximity labeling strategy that exploits LD-targeted APEX2 to biotinylate LD proteins in living cells. Application of this approach to U2OS and Huh7 cells identified the vast majority of previously validated LD proteins, excluded common contaminating proteins, and revealed new LD proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2017-12-22 |
AnnouncementXML | Submission_2018-05-23_07:23:13.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | James Olzmann |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotinylated residue |
Instrument | 6410 Triple Quadrupole LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-09-08 05:49:56 | ID requested | |
1 | 2017-12-22 00:14:17 | announced | |
⏵ 2 | 2018-05-23 07:23:14 | announced | Updated publication reference for PubMed record(s): 29275994. |
Publication List
Bersuker K, Peterson CWH, To M, Sahl SJ, Savikhin V, Grossman EA, Nomura DK, Olzmann JA, A Proximity Labeling Strategy Provides Insights into the Composition and Dynamics of Lipid Droplet Proteomes. Dev Cell, 44(1):97-112.e7(2018) [pubmed] |
Keyword List
curator keyword: Technical, Biological |
submitter keyword: Lipid droplet, APEX, ERAD |
Contact List
James Olzmann |
contact affiliation | University of California, Berkeley |
contact email | olzmann@berkeley.edu |
lab head | |
James Olzmann |
contact affiliation | University of California, Berkeley |
contact email | jolzmann@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD007695 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007695
- Label: PRIDE project
- Name: Lipid droplet-targeted APEX proteome