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PXD007686

PXD007686 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative proteomics of dying and surviving cancer cells improves the identification of drug targets and sheds light on cell life/death decisions
DescriptionChemotherapeutics cause the detachment and death of adherent cancer cells. When studying the proteome changes to determine the protein target and mechanism of action of anticancer drugs, the still-attached cells are normally used, while the detached cells are usually ignored. Here we tested the hypothesis that proteomes of detached cells contain valuable information and therefore separately analyzed the proteomes of detached and attached HCT-116, A375 and RKO cells obtained 48 h after treatment with 5-fluorouracil, methotrexate and paclitaxel. Combined proteome data provided a more accurate identification of drug targets. Six proteins consistently up- or down-regulated in the detached vs attached cells regardless of the drug and cell type were targeted by siRNA. Knocking down USP11, CTTN, ACAA2 and EIF4H had anti-proliferative effects, targeting UHRF1 additionally sensitized the cells to the anticancer drugs, while knocking down RNF-40 increased cell survival against the treatments. Therefore, these proteins are likely to be involved in general cell death and survival decisions. Adding detached cells to the analysis could become a standard practice in expression proteomics of drug-treated cells.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:40:22.473.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAmir Ata Saei
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-09-07 02:27:29ID requested
12018-03-28 07:18:37announced
22024-10-22 04:40:23announced2024-10-22: Updated project metadata.
Publication List
Saei AA, Sabatier P, Tokat Ü G, Chernobrovkin A, Pirmoradian M, Zubarev RA, Comparative Proteomics of Dying and Surviving Cancer Cells Improves the Identification of Drug Targets and Sheds Light on Cell Life/Death Decisions. Mol Cell Proteomics, 17(6):1144-1155(2018) [pubmed]
10.1074/mcp.ra118.000610;
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: cell death, mass spectrometry, survival, drug mechanism, chemotherapeutic
Contact List
Roman Zubarev
contact affiliationDepartment of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
contact emailroman.zubarev@ki.se
lab head
Amir Ata Saei
contact affiliationDepartment of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
contact emailamirata.saei.dibavar@ki.se
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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