PXD007684

PXD007684 is an original dataset announced via ProteomeXchange.

Title	Cell Surface Capture of mouse PSC-derived Cardiac Progenitor Cells
Description	The identification of cell surface proteins on stem cells or stem cell derivatives is a key strategy for the functional characterization, isolation, and understanding of stem cell population dynamics. Here, using an integrated mass spectrometry and microarray based approach, we analyzed the surface proteome and transcriptome of cardiac progenitor cells (CPCs) generated from the stage-specific differentiation of mouse and human pluripotent stem cells. Through bioinformatic analysis, we have identified and characterized FZD4 as a new marker for lateral plate mesoderm. Additionally, we utilized FZD4, in conjunction with FLK1 and PDGFRA, to further purify CPCs and increase cardiomyocyte (CM) enrichment in both mouse and human systems. Moreover, we have shown that NORRIN presented to FZD4 further increases CM output via proliferation through the canonical WNT pathway. Taken together, these findings demonstrate a role for FZD4 in mammalian cardiac development.
HostingRepository	PRIDE
AnnounceDate	2018-01-03
AnnouncementXML	Submission_2018-01-03_00:35:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Zandstra
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-07 02:10:08	ID requested
⏵ 1	2018-01-03 00:35:14	announced

Yoon C, Song H, Yin T, Bausch-Fluck D, Frei AP, Kattman S, Dubois N, Witty AD, Hewel JA, Guo H, Emili A, Wollscheid B, Keller G, Zandstra PW, FZD4 Marks Lateral Plate Mesoderm and Signals with NORRIN to Increase Cardiomyocyte Induction from Pluripotent Stem Cell-Derived Cardiac Progenitors. Stem Cell Reports, 10(1):87-100(2018) [pubmed]

curator keyword: Biological

submitter keyword: mouse, pluripotent stem cell, cardiac progenitor cell, mass spectrometry

Peter Zandstra
contact affiliation	IBBME, Stem Cell Bioengineering, University of Toronto, Canada
contact email	peter.zandstra@utoronto.ca
lab head
Peter Zandstra
contact affiliation	University of Toronto
contact email	peter.zandstra@utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD007684

PRIDE project URI

• PRIDE
  1. PXD007684
     1. Label: PRIDE project
     2. Name: Cell Surface Capture of mouse PSC-derived Cardiac Progenitor Cells