PXD007675

PXD007675 is an original dataset announced via ProteomeXchange.

Title	Molecular basis of Tousled-Like-Kinase 2 (TLK2) activation – LC-MS/MS-based analysis of TLK2 phosphorylation state
Description	In this study on human Tousled-Like-Kinase 2 (TLK2) mass spectrometry-based analysis was applied as part of a molecular characterization of TLK2 in the aim to increase the understanding of the mode of activation of this protein. Specifically, different TLK2 constructs were analyzed to generate a “phosphorylation map” of the TLK2 protein. To analyse the phosphorylation state of TLK2 we used recombinant protein purified from Escherichia coli. Samples of different TLK2 constructs were used for in-gel digestion and analysed by mass spectrometry. All samples were prepared and run in triplicates for label-free quantification. Explanation for MS raw files with TLK2 construct included: Ac: TLK2 construct consisting of amino acid residues 191-772 (Ac indicates active form). KD: TLK2 construct consisting of amino acid residues 191-772 with mutation D613A (KD indicates kinase-dead form). FusionKD: Kinase-dead part of a heterodimeric TLK2 construct (amino acid residues 191-772) with mutation D613A (KD indicates kinase-dead form). FusionAC: Active part of a heterodimeric TLK2 construct (amino acid residues 191-772) (Ac indicates kinase-dead form). KDlong: Kinase domain of TLK2 with C-tail (amino acid residues 450-772). KDshort: Kinase domain of TLK2 without C-tail (amino acid residues 450-753).
HostingRepository	PRIDE
AnnounceDate	2018-10-23
AnnouncementXML	Submission_2018-10-23_14:57:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna-Kathrine Pedersen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-06 07:22:13	ID requested
⏵ 1	2018-10-23 14:57:57	announced

Mortuza GB, Hermida D, Pedersen AK, Segura-Bayona S, L, ó, pez-M, é, ndez B, Redondo P, R, ü, ther P, Pozdnyakova I, Garrote AM, Mu, ñ, oz IG, Villamor-Pay, à M, Jauset C, Olsen JV, Stracker TH, Montoya G, Molecular basis of Tousled-Like Kinase 2 activation. Nat Commun, 9(1):2535(2018) [pubmed]

curator keyword: Biological

submitter keyword: Human, TLK2, LC-MSMS

Guillermo Montoya
contact affiliation	Macromolecular Crystallography Group, NNF Centre for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, Copenhagen 2200, Denmark
contact email	guillermo.montoya@cpr.ku.dk
lab head
Anna-Kathrine Pedersen
contact affiliation	NNF Center for Protein Research, University of Copenhagen
contact email	anna-kathrine.pedersen@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007675

PRIDE project URI

• PRIDE
  1. PXD007675
     1. Label: PRIDE project
     2. Name: Molecular basis of Tousled-Like-Kinase 2 (TLK2) activation – LC-MS/MS-based analysis of TLK2 phosphorylation state