PXD007673

PXD007673 is an original dataset announced via ProteomeXchange.

Title	Chemical cross-linking mass spectrometry for systems structural biology in mouse heart tissue.
Description	While modern structural biology technologies have greatly expanded the size and type of protein complexes that can now be studied, the ability to derive large-scale structural information on proteins and complexes as they exist within tissues is practically non-existent. Many protein properties and their involvement in disease pathways are not replicated in cell culture and some complexes are not amenable to purification. Thus, the lack of tissue-level structural information limits molecular-level insight on diseases such as heart failure. Here we demonstrate the application of XL-MS to identify protein structural features and interactions in tissue samples, providing the first systems structural biology insight on protein complexes as they exist in the mouse heart.
HostingRepository	PRIDE
AnnounceDate	2017-11-30
AnnouncementXML	Submission_2017-12-11_03:58:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Chavez
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	LTQ FT; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-06 05:37:24	ID requested
1	2017-11-30 00:56:19	announced
⏵ 2	2017-12-11 03:58:11	announced	Updated publication reference for PubMed record(s): 29199018.

Chavez JD, Lee CF, Caudal A, Keller A, Tian R, Bruce JE, Chemical Crosslinking Mass Spectrometry Analysis of Protein Conformations and Supercomplexes in Heart Tissue. Cell Syst, 6(1):136-141.e5(2018) [pubmed]

curator keyword: Biological

submitter keyword: Mouse, Heart, Cross-linking, Mass Spectrometry, Structural Biology, Protein-Protein Interactions

James E. Bruce
contact affiliation	Genome Sciences, University of Washington, USA
contact email	jimbruce@uw.edu
lab head
Juan Chavez
contact affiliation	University of Washington
contact email	jdchavez@uw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD007673

PRIDE project URI

• PRIDE
  1. PXD007673
     1. Label: PRIDE project
     2. Name: Chemical cross-linking mass spectrometry for systems structural biology in mouse heart tissue.