PXD007669

PXD007669 is an original dataset announced via ProteomeXchange.

Title	Non-canonical circadian oscillations in Drosophila S2 cells
Description	Circadian rhythms are cell-autonomous biological oscillations with a period of about 24 hours. Current models propose that transcriptional feedback loops are the principal mechanism for the generation of circadian oscillations. In these models, Drosophila S2 cells are generally regarded as ‘non-rhythmic’ cells, as they do not express several canonical circadian components. Using an unbiased multi-omics approach, we made the surprising discovery that Drosophila S2 cells do in fact display widespread daily rhythms. Transcriptomics and proteomics analyses revealed that hundreds of genes and their products are rhythmically expressed in a 24-hour cycle. Metabolomics analyses extended these findings and illustrated that central carbon metabolism and amino acid metabolism are the main pathways regulated in a rhythmic fashion. We thus demonstrate that daily genome-wide oscillations, coupled to metabolic cycles, take place in eukaryotic cells without the contribution of known circadian regulators.
HostingRepository	PRIDE
AnnounceDate	2018-07-31
AnnouncementXML	Submission_2018-07-31_06:30:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillaume Rey
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-06 03:42:34	ID requested
⏵ 1	2018-07-31 06:30:07	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Circadian, clock, LC-MS, TMT

Akhilesh B. Reddy
contact affiliation	The Francis Crick Institute, London
contact email	Akhilesh.Reddy@crick.ac.uk
lab head
Guillaume Rey
contact affiliation	University of Cambridge
contact email	guiomrey@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD007669

PRIDE project URI

• PRIDE
  1. PXD007669
     1. Label: PRIDE project
     2. Name: Non-canonical circadian oscillations in Drosophila S2 cells