PXD007664

PXD007664 is an original dataset announced via ProteomeXchange.

Title	A Spatial Interactome Reveals the Protein Organization of the Algal CO2 Concentrating Mechanism
Description	One-third of global CO2 fixation is mediated by eukaryotic algae. Nearly all algae enhance their carbon assimilation by operating a CO2 concentrating mechanism (CCM), built around a mysterious organelle called the pyrenoid. Here, we leveraged new tools to determine the localizations of 146 candidate CCM proteins in the model alga Chlamydomonas reinhardtii. Twelve of the tagged proteins localized to the pyrenoid in six distinct sub-pyrenoid localizations: matrix, membrane tubules, plates, a mesh layer, and two punctate layers. Many stromal proteins can access the pyrenoid matrix. The proteins with access to the matrix tend to be small, suggesting that the pyrenoid may show protein size selectivity. Contrary to current models, the carbonic anhydrase CAH6 is in the flagella. Affinity purification mass spectrometry of 38 CCM-related proteins reveals dozens of additional components, including novel Rubisco interacting proteins, photosystem I assembly factor candidates and inorganic carbon flux components. Together, our spatial interactome reveals new protein components, sub-compartments and biophysical characteristics that open doors to a detailed molecular characterization of the algal CCM.
HostingRepository	PRIDE
AnnounceDate	2018-01-24
AnnouncementXML	Submission_2018-01-24_02:43:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ryan Leib
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-05 08:28:42	ID requested
⏵ 1	2018-01-24 02:44:00	announced

Mackinder LCM, Chen C, Leib RD, Patena W, Blum SR, Rodman M, Ramundo S, Adams CM, Jonikas MC, -Concentrating Mechanism. Cell, 171(1):133-147.e14(2017) [pubmed]

curator keyword: Biological

submitter keyword: chlamydomonas reinhardtii, interactome, orbitrap, 15N isotopic labelling, CO2-concentrating mechanism, CCM, carbon-fixation, photosynthesis, pyrenoid, Rubisco, high-throughput fluorescence protein tagging, affinity purification mass spectrometry

Martin C Jonikas
contact affiliation	Department of Molecular Biology, Princeton University, Princeton, NJ, USA
contact email	mjonikas@princeton.edu
lab head
Ryan Leib
contact affiliation	Stanford University
contact email	rdleib@stanford.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007664
     1. Label: PRIDE project
     2. Name: A Spatial Interactome Reveals the Protein Organization of the Algal CO2 Concentrating Mechanism