PXD007655

PXD007655 is an original dataset announced via ProteomeXchange.

Title	A Comparative Proteomic Analysis of Whey Protein from Early, Mid and Late Lactation Stages of Indian Indigenous Breed, MalnadGidda (Bosindicus)
Description	Different lactation stages have marked influence on milk yield, milk constituents and nourishment of the neonates. However, the differential gene expression during different lactation stages in Bosindicus has not been investigated so far. In this study, we carried out high-resolution mass spectrometry-based quantitative proteomics of bovine whey at early, mid and late lactation stages of MalnadGidda (Bosindicus) cows. Using TMT-based quantitative proteomics, we compared the bovine whey proteins on progressive lactation stages of Indian breed, MalnadGidda(Bosindicus). LC-MS/MS analysis of whey peptides from early, mid and late lactation stages resulted in the generation of 420,092 MS/MS spectra and 50,800 peptide spectrum matches, which led to the identification of 4,450 peptides corresponding to 725 proteins. Out of which, 440 proteins were differentially expressed (≥1.5-fold). Gene Ontology studies showed that proteins that regulatemilk composition and mammary growth associated proteins are abundantly expressed during peak lactation stages. Whereas, proteins related to pregnancy and mammary involution are expressed high in late and mid lactation stages indicating the physiological changes in the maternal system of bovine during drying period. Detection of progestagen associated endometrial protein; an immune protein seen in the fetomaternal interface and other pregnancy associated proteins at mid lactation suggest a candidate biomarker for the early pregnancy diagnosis. These results are overlapping with the previous findings addressed in milk from exotic breeds. We strongly believe that this preliminary investigation on differential proteome in milk whey over the course of lactation of indigenous cattle could answer many unsolved questions in lactation biology.
HostingRepository	PRIDE
AnnounceDate	2018-02-16
AnnouncementXML	Submission_2018-02-16_02:36:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keshava Prasad T. S.
SpeciesList	scientific name: Bos indicus; NCBI TaxID: 9915;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-05 01:23:27	ID requested
⏵ 1	2018-02-16 02:36:52	announced

Mol P, Kannegundla U, Dey G, Gopalakrishnan L, Dammalli M, Kumar M, Patil AH, Basavaraju M, Rao A, Ramesha KP, Prasad TSK, Bovine Milk Comparative Proteome Analysis from Early, Mid, and Late Lactation in the Cattle Breed, Malnad Gidda (Bos indicus). OMICS, 22(3):223-235(2018) [pubmed]

curator keyword: Biological

submitter keyword: bRPLC, biomarker, ultracentrifugation, isoelectric precipitation, TMT labeling

T. S. Keshava Prasad
contact affiliation	T. S. Keshava Prasad, Institute of Bioinformatics, International Technology Park, Bangalore 560066, India
contact email	keshav@ibioinformatics.org
lab head
Keshava Prasad T. S.
contact affiliation	Institute of Bioinformatics, Bangalore, Karnataka, India.
contact email	keshav@ibioinformatics.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007655
     1. Label: PRIDE project
     2. Name: A Comparative Proteomic Analysis of Whey Protein from Early, Mid and Late Lactation Stages of Indian Indigenous Breed, MalnadGidda (Bosindicus)