PXD007653

PXD007653 is an original dataset announced via ProteomeXchange.

Title	Proteomics identifies organelle specific phosphorylation and reveals major subcellular reorganization in the progression of NAFLD
Description	Obesity induced non-alcoholic fatty liver disease (NAFLD) is associated with the development of type 2 diabetes and constitutes a major health problem. A hallmark of the diseases is extensive lipid droplet (LD) formation and accumulation of toxic lipid species interfering with cellular signaling and functions. However, the cellular mechanisms during disease progression and cellular lipid overflow are poorly understood. Here, we develop a novel workflow for label free mass spectrometry based protein and phosphopeptide correlation profiling to systematically monitor the level and cellular distribution of ~6000 liver proteins and ~16,000 phosphosites during the development of dietary induced steatosis in mice. We see a redistribution of the secretory apparatus including a mislocalization of the COPI complex, and targeting of all analyzed Golgi apparatus proteins to LDs what leads to a general reduction of hepatic protein secretion. Further, we identify targeting of several organelle contact site proteins to LDs, accompanied by increased contacts between LDs and other organelles. Our resource provides the first systematic in vivo analysis of subcellular re-arrangements and organelle specific phosphorylation during disease development.
HostingRepository	PRIDE
AnnounceDate	2018-10-29
AnnouncementXML	Submission_2018-10-30_08:03:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natalie Krahmer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-04 23:29:17	ID requested
1	2018-10-29 11:46:14	announced
⏵ 2	2018-10-30 08:03:09	announced	Updated publication reference for PubMed record(s): 30352176.

Krahmer N, Najafi B, Schueder F, Quagliarini F, Steger M, Seitz S, Kasper R, Salinas F, Cox J, Uhlenhaut NH, Walther TC, Jungmann R, Zeigerer A, Borner GHH, Mann M, Organellar Proteomics and Phospho-Proteomics Reveal Subcellular Reorganization in Diet-Induced Hepatic Steatosis. Dev Cell, 47(2):205-221.e7(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Diabetes, Steatosis, organelle protoemics, liver,

Matthias Mann
contact affiliation	Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
contact email	mmann@biochem.mpg.de
lab head
Natalie Krahmer
contact affiliation	Max Planck of Biochemistry
contact email	krahmer@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007653

PRIDE project URI

• PRIDE
  1. PXD007653
     1. Label: PRIDE project
     2. Name: Proteomics identifies organelle specific phosphorylation and reveals major subcellular reorganization in the progression of NAFLD