PXD007650

PXD007650 is an original dataset announced via ProteomeXchange.

Title	ATP MODIFIES THE PROTEOME OF EXTRACELLULAR VESICLES RELEASED BY MICROGLIA AND INFLUENCES THEIR ACTION ON ASTROCYTES
Description	Extracellular ATP is among molecules promoting microglia activation and inducing the release of extracellular vesicles (EVs), which are potent mediators of intercellular communication between microglia and the microenvironment. We previously showed that EVs produced under ATP stimulation (ATP-EVs) propagate a robust inflammatory reaction among astrocytes and microglia in vitro and in mice with subclinical neuroinflammation. However, the proteome of EVs released upon ATP stimulation has not yet been elucidated. In this study we applied a label free proteomic approach to characterize the proteome of EVs released constitutively and during microglia activation with ATP. We show that ATP drives sorting in EVs of a set of proteins implicated in cell adhesion/extracellular matrix organization, autophagy-lysosomal pathway and cellular metabolism, that may influence the response of recipient astrocytes to EVs. These data provide new clues to molecular mechanisms involved in microglia response to ATP and in microglia signalling to the environment via EVs.
HostingRepository	PRIDE
AnnounceDate	2018-10-23
AnnouncementXML	Submission_2018-10-23_15:12:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julien Franck
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-04 07:30:09	ID requested
⏵ 1	2018-10-23 15:12:45	announced

Drago F, Lombardi M, Prada I, Gabrielli M, Joshi P, Cojoc D, Franck J, Fournier I, Vizioli J, Verderio C, ATP Modifies the Proteome of Extracellular Vesicles Released by Microglia and Influences Their Action on Astrocytes. Front Pharmacol, 8():910(2017) [pubmed]

curator keyword: Biological

submitter keyword: ATP, microglia, extracellular vesicles, proteomics, astrocyte activation

Isabelle Fournier
contact affiliation	Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM) Inserm U1192, Université de Lille Sciences et Technologies, Villeneuve d'Ascq, France.
contact email	isabelle.fournier@univ-lille1.fr
lab head
Julien Franck
contact affiliation	PRISM-INSERM U1192
contact email	julien.franck@univ-lille1.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007650

PRIDE project URI

• PRIDE
  1. PXD007650
     1. Label: PRIDE project
     2. Name: ATP MODIFIES THE PROTEOME OF EXTRACELLULAR VESICLES RELEASED BY MICROGLIA AND INFLUENCES THEIR ACTION ON ASTROCYTES