PXD007646
PXD007646 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Serial-Omics of Equine Urine - Serial-omics characterization of equine urine |
Description | Horse urine is easily collected and contains molecules readily measurable using mass spectrometry that can be used as biomarkers representative of health, disease or drug tampering. This study aimed at analyzing microliter levels of horse urine to purify, identify and quantify proteins, polar metabolites and non-polar lipids. Urine from a healthy 12 year old quarter horse mare on a diet of grass hay and vitamin/mineral supplements with limited pasture access was collected for serial-omics characterization. The urine was treated with methyl tert-butyl ether (MTBE) and methanol to partition into three distinct layers for protein, non-polar lipid and polar metabolite content from a single liquid-liquid extraction and was repeated two times. Each layer was analyzed by high performance liquid chromatography – high resolution tandem mass spectrometry (LC-MS/MS) to obtain protein sequence and relative protein levels as well as identify and quantify small polar metabolites and lipids. The results show 46 urine proteins, many related to normal kidney function, structural and circulatory proteins as well as 474 small polar metabolites but only 10 lipid molecules. Metabolites were mostly related to urea cycle and ammonia recycling as well as amino acid related pathways, plant diet specific molecules, etc. The few lipids represented triglycerides and phospholipids. These data show a complete mass spectrometry based –omics characterization of equine urine from a single 333 uL mid-stream urine aliquot. The data can be used as a baseline for healthy urine composition and analyses can be used to monitor disease progression, health status, monitor drug use, etc. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:40:11.788.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | John Asara |
SpeciesList | scientific name: Equus caballus (Horse); NCBI TaxID: 9796; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q TRAP; Q Exactive; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2017-09-04 05:59:45 | ID requested | |
1 | 2018-07-23 08:25:28 | announced | |
⏵ 2 | 2024-10-22 04:40:12 | announced | 2024-10-22: Updated project metadata. |
Publication List
Yuan M, Breitkopf SB, Asara JM, Serial-omics characterization of equine urine. PLoS One, 12(10):e0186258(2017) [pubmed] |
10.1371/journal.pone.0186258; |
Keyword List
curator keyword: Biological |
submitter keyword: Metabolomics, Equine, Lipidomics,Horse, Proteomics, Urine, Integration, Biomarkers |
Contact List
John M Asara | |
---|---|
contact affiliation | Associate Professor of Medicine Harvard Medical School Director, Mass Spectrometry Core Beth Israel Deaconess Medical Center 3 Blackfan Circle CLS 447 office CLS 425 lab Boston, MA 02115 Tel: 617-735-2651 |
contact email | jasara@bidmc.harvard.edu |
lab head | |
John Asara | |
contact affiliation | Signal Transduction |
contact email | jasara@bidmc.harvard.edu |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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