PXD007644

PXD007644 is an original dataset announced via ProteomeXchange.

Title	Proteomic dissection of substrate nanotopography-sensitive mechanotransductive signalling hubs that foster neuronal differentiation.
Description	Neuronal cells are competent in precisely sensing nanotopographical features of their microenvironment and the intrinsic information impacts on neuronal functioning and differentiation “interpreted” by mechanotransductive processes. Attempts to influence and direct neuronal differentiation behaviour by engineering substrates that mimic appropriate extracellular matrix (ECM) topographies are hampered by the difficulty since many details of mechanosensing/-transduction are still elusive. Introducing omics methods into these biomaterial approaches has the potential to provide a deeper insight into the molecular processes and signalling cascades underlying mechanosensing/-transduction but their exigence in cellular material is often opposed by technical limitations of the biomaterial fabrication methods. Supersonic cluster beam deposition (SCBD) of zirconia nanoparticles allows instead the realisation of large macroscopic areas with biocompatible nanostructured films equipped with controllable and reproducible ECM-like nanoroughness that have been recently shown to foster neuron differentiation and maturation. Exploiting this capacity of SCBD, we challenged mechanosensing/-transduction and differentiative behaviour of neuron-like PC12 cells interacting with diverse nanotopographies and/or by changing their biomechanical status, to analyse their phosphoproteomic profiles in these settings. Versatile proteins were found to be affected that can be associated to significant processes along the mechanotransductive signal sequence, i.e. cell/cell interaction, glycocalyx and ECM, membrane/f-actin linkage and integrin activation, cell/substrate interaction, IAC, actomyosin organisation/cellular mechanics, nuclear organisation and transcription. The phosphoproteomic data suggested furthermore an involvement of ILK, mTor, Wnt and calcium signalling in these nanotopography- and/or cell mechanics-related processes. Altogether, potential nanotopography-sensitive, mechanotransductive signalling hubs participating to neuronal differentiation were dissected.
HostingRepository	PRIDE
AnnounceDate	2018-01-24
AnnouncementXML	Submission_2018-10-08_03:52:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	gabriella tedeschi
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	L-methionine sulfoxide; phosphorylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-04 05:22:09	ID requested
1	2018-01-25 01:32:30	announced
⏵ 2	2018-10-08 03:52:44	announced	Updated publication reference for PubMed record(s): 30290237, 29354032.

Maffioli E, Schulte C, Nonnis S, Grassi Scalvini F, Piazzoni C, Lenardi C, Negri A, Milani P, Tedeschi G, Proteomic Dissection of Nanotopography-Sensitive Mechanotransductive Signaling Hubs that Foster Neuronal Differentiation in PC12 Cells. Front Cell Neurosci, 11():417(2017) [pubmed]

Tripodi F, Castoldi A, Nicastro R, Reghellin V, Lombardi L, Airoldi C, Falletta E, Maffioli E, Scarcia P, Palmieri L, Alberghina L, Agrimi G, Tedeschi G, Coccetti P, Methionine supplementation stimulates mitochondrial respiration. Biochim Biophys Acta Mol Cell Res, 1865(12):1901-1913(2018) [pubmed]

curator keyword: Biological

submitter keyword: biomaterial, mechanotransduction, cell mechanics, biophysics, PC12 cells, quantitative proteomics, shot gun proteomics, integrin signalling, zirconia nanosubstrates, neuronal differentiation, NGF

Gabriella Tedeschi
contact affiliation	Dipartimento di Medicina Veterinaria, Università degli Studi di Milano, Milano; Italy
contact email	gabriella.tedeschi@unimi.it
lab head
gabriella tedeschi
contact affiliation	University of Milano
contact email	gabriella.tedeschi@unimi.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD007644

PRIDE project URI

• PRIDE
  1. PXD007644
     1. Label: PRIDE project
     2. Name: Proteomic dissection of substrate nanotopography-sensitive mechanotransductive signalling hubs that foster neuronal differentiation.