PXD007640

PXD007640 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics and bioinformatics analyses reveal the mechanism of pan-aurora kinase inhibitor tozasertib in neuroblastoma
Description	Neuroblastoma is the third most common pediatric cancer and is responsible for approximately 15% of all childhood cancer deaths (Maris & Matthay, 1999). In our analysis, we found that poor patient survival with increasing mRNA expression level of AURKA and AURKB in Mycn-amplified neuroblastoma. In the light of this evidence, we were able to find possibilities of existing inhibitors for therapy. According to the following experiments, we found that tozasertib, a pan-Aurora kinase inhibitor, has high therapeutic potential in neuroblastoma treatment. First, we performed in vitro experiments to reveal that tozasertib suppressed cell proliferation in multiple Mycn-amplified neuroblastoma cell lines. Next, we evaluated ex vivo not only in Mycn-amplified neuroblastoma xenograft mouse model but also TH-Mycn transgenic mouse model. The results showed that tozasertib significantly inhibited the tumor growth and prolonged the survival probability in both animal models. Finally, we explored the mechanism of tozasertib-treated tissues in two animal models by iTRAQ proteomic.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:40:21.414.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiao-Hui Hsieh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-04 01:52:21	ID requested
1	2019-09-30 02:08:54	announced
2	2019-10-07 00:24:36	announced	2019-10-07: Updated publication reference for PubMed record(s): 31560547.
⏵ 3	2024-10-22 04:40:29	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.9b00245;

Hsieh CH, Cheung CHY, Liu YL, Hou CL, Hsu CL, Huang CT, Yang TS, Chen SF, Chen CN, Hsu WM, Huang HC, Juan HF, Quantitative Proteomics of Th-MYCN Transgenic Mice Reveals Aurora Kinase Inhibitor Altered Metabolic Pathways and Enhanced ACADM To Suppress Neuroblastoma Progression. J Proteome Res, 18(11):3850-3866(2019) [pubmed]

submitter keyword: Neuroblastoma/ tozasertib/ iTRAQ/ Metabolite/ aurora kinase inhibitor.

Hsueh-Fen Juan
contact affiliation	Institute of Molecular and Cellular Biology, Department of Life Science, National Taiwan University, Taipei, Taiwan
contact email	yukijuan@ntu.edu.tw
lab head
Chiao-Hui Hsieh
contact affiliation	Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taiwan
contact email	ab032808@hotmail.com.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007640
     1. Label: PRIDE project
     2. Name: Quantitative proteomics and bioinformatics analyses reveal the mechanism of pan-aurora kinase inhibitor tozasertib in neuroblastoma