PXD007629 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterisation of the biflavonoid hinokiflavone as a pre-mRNA splicing modulator that inhibits SENP |
Description | We have identified the plant biflavonoid hinokiflavone as an inhibitor of splicing in vitro and modulator of alternative splicing in cells. Chemical synthesis confirms hinokiflavone is the active molecule. Hinokiflavone inhibits splicing in vitro by blocking spliceosome assembly, preventing formation of the B complex. Cells treated with hinokiflavone show altered subnuclear organization specifically of splicing factors required for A complex formation, which relocalize together with SUMO1 and SUMO2 into enlarged nuclear speckles containing polyadenylated RNA. Hinokiflavone increases protein SUMOylation levels, both in in vitro splicing reactions and in cells. Hinokiflavone also inhibited a purified, E. coli expressed SUMO protease, SENP1, in vitro, indicating the increase in SUMOylated proteins results primarily from inhibition of de-SUMOylation. Using a quantitative proteomics assay we identified many SUMO2 sites whose levels increased in cells following hinokiflavone treatment, with the major targets including 6 proteins that are components of the U2 snRNP and required for A complex formation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:40:16.351.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andrea Pawellek |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); ubiquitination signature dipeptidyl lysine; phosphorylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-09-01 06:34:46 | ID requested | |
1 | 2017-09-07 06:48:35 | announced | |
2 | 2017-09-12 02:05:49 | announced | Updated publication reference for PubMed record(s): 28884683. |
⏵ 3 | 2024-10-22 04:40:24 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.7554/elife.27402; |
Pawellek A, Ryder U, Tammsalu T, King LJ, Kreinin H, Ly T, Hay RT, Hartley RC, Lamond AI, Characterisation of the biflavonoid hinokiflavone as a pre-mRNA splicing modulator that inhibits SENP. Elife, 6():(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Human embryonic kidney (HEK) suspension cells, SUMO2 |
Contact List
Angus Iain Lamond |
contact affiliation | Gene expression and Regulation, School of Life Sciences, University of Dundee, UK |
contact email | a.i.lamond@dundee.ac.uk |
lab head | |
Andrea Pawellek |
contact affiliation | University of Dundee |
contact email | a.pawellek@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007629
- Label: PRIDE project
- Name: Characterisation of the biflavonoid hinokiflavone as a pre-mRNA splicing modulator that inhibits SENP