PXD007628

PXD007628 is an original dataset announced via ProteomeXchange.

Title	MHC II immunopeptidome during Plasmodium berghei blood stage infection
Description	In malaria, T cells play a dual role by both restricting parasite growth and mediating immunopathology such as the deadly neuroinflammation called cerebral malaria. During experimental cerebral malaria (ECM), IFN produced by CD4 T cells promotes CD8 T cell sequestration in brain capillaries, resulting in endothelial damage, oedema and death. However the antigen-presenting cells controlling the development of CD4 T cell responses, as well as the antigens recognized by these CD4 T cells, are unknown. Here we used mass spectrometry to characterize the MHC II immunopeptidome presented by dendritic cells during blood stage malaria in C57BL/6 mice. We identified 14 MHC II ligands derived from 13 conserved Plasmodium berghei proteins that we validated in vivo. This work profiles the first MHC II immunopeptidome in a mouse model of blood stage malaria.
HostingRepository	PRIDE
AnnounceDate	2017-09-20
AnnouncementXML	Submission_2018-05-23_05:59:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SALIOU Jean-Michel
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-01 05:50:19	ID requested
1	2017-09-20 02:41:50	announced
⏵ 2	2018-05-23 05:59:26	announced	Updated publication reference for PubMed record(s): 28935714.

Draheim M, Wlodarczyk MF, Crozat K, Saliou JM, Alayi TD, Tomavo S, Hassan A, Salvioni A, Demarta-Gatsi C, Sidney J, Sette A, Dalod M, Berry A, Silvie O, Blanchard N, Profiling MHC II immunopeptidome of blood-stage malaria reveals that cDC1 control the functionality of parasite-specific CD4 T cells. EMBO Mol Med, 9(11):1605-1621(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Mouse, MHC II, Plasmodium berghei, malaria, LC-MSMS

Jean-Michel Saliou
contact affiliation	Centre d'Infection et d'Immunité de Lille Univ. Lille (CIIL), Univ. Lille, CNRS UMR 8204, Inserm U1019, CHU Lille, Institut Pasteur de Lille, F-59000 Lille, France.
contact email	Jean-Michel.Saliou@pasteur-lille.fr
lab head
SALIOU Jean-Michel
contact affiliation	Institut Pasteur de Lille
contact email	jean-michel.saliou@pasteur-lille.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007628
     1. Label: PRIDE project
     2. Name: MHC II immunopeptidome during Plasmodium berghei blood stage infection