PXD007615

PXD007615 is an original dataset announced via ProteomeXchange.

Title	Cisplatin-induced proteome changes in ovatian cancer cells
Description	Tumors easily acquire resistance to individual drugs and drug combinations thereby limiting the treatment effectiveness. Drug-stressed tumor cells secrete components that promote the survival of recipient cells and repopulation of damaged tumors. We have previously found that therapy-induced secretomes are enriched with spliceosomal proteins. The behavior of splicing-associated genes in response to chemotherapeutic drugs is poorly understood. We show that a severely stressed cell dramatically down-regulates spliceosomal proteins by secreting them into the extracellular space, reducing the expression of the respective genes, and disrupting the splicing. Our meta-analysis indicates that drugs with different action mechanisms cause a correlated decrease in the transcription of genes involved in spliceosome and mitotic cell cycle pathways and induce similar intron retention patterns in these genes. The key transcription factors regulating the concerted change in transcription are MYC, E2F1, TP53, and WT1. Based on the correlation analysis with siRNA data, we conclude that a decrease in the representation of intracellular spliceosomal proteins in response to stress is aimed at the cell cycle arrest. At the expression and processing levels, we demonstrate that a strong interplay between splicing-associated and cell cycle genes develops in stressed cancer cells.
HostingRepository	PRIDE
AnnounceDate	2018-05-22
AnnouncementXML	Submission_2018-05-22_05:27:02.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007615
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Georgij Arapidi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Ammonia-loss; Dehydrated; Oxidation; Acetyl; Carbamidomethyl
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-31 02:52:29	ID requested
⏵ 1	2018-05-22 05:27:02	announced

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Chemotherapy, DNA damage response, Spliceosome

Georgij Pavlovich Arapidi
contact affiliation	Laboratory of Proteomics, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
contact email	arapidi@gmail.com
lab head
Georgij Arapidi
contact affiliation	IBCH RAS
contact email	arapidi@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007615
     1. Label: PRIDE project
     2. Name: Cisplatin-induced proteome changes in ovatian cancer cells