PXD007612

PXD007612 is an original dataset announced via ProteomeXchange.

Title	Maize and Arabidopsis Golgi proteome
Description	Comparative proteomics of ER-Golgi membranes from etiolated coleoptiles of maize (Zea mays) and young leaves of Arabidopsis (Arabidopsis thaliana) isolated by flotation centrifugation demonstrate differences in the complement of synthases and glycosyl transferases unique to the type of wall made. Additionally, maize Golgi membranes enriched by flotation were separated further by free-flow electrophoresis (FFE), yielding 3009 proteins, of which 229 were known to function in cell wall synthesis or metabolism. A subset of proteins identified after flotation centrifugation were identified in Golgi membranes but failed to enrich in them. Individual classes of proteins associated with cell wall synthesis were asymmetrically distributed across the fractions of Golgi membranes separated by FFE.
HostingRepository	PRIDE
AnnounceDate	2019-04-11
AnnouncementXML	Submission_2019-07-08_00:19:57.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007612
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ikenna Okekeogbu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	Oxidation; Acetyl; Ethanolyl
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-31 01:25:50	ID requested
1	2019-04-11 04:09:18	announced
⏵ 2	2019-07-08 00:19:58	announced	Updated publication reference for PubMed record(s): 30914498.

Okekeogbu IO, Pattathil S, Gonz, á, lez Fern, á, ndez-Ni, ñ, o SM, Aryal UK, Penning BW, Lao J, Heazlewood JL, Hahn MG, McCann MC, Carpita NC, Glycome and Proteome Components of Golgi Membranes Are Common between Two Angiosperms with Distinct Cell-Wall Structures. Plant Cell, 31(5):1094-1112(2019) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis, maize, Golgi, flotation centrifugation, Free-Flow Electrophoresis, FFE, LC-MSMS, Triple TOF

Nicholas Carpita
contact affiliation	Carpita Lab; Department of Botany & Plant Pathology, Purdue University, USA
contact email	carpita@purdue.edu
lab head
Ikenna Okekeogbu
contact affiliation	Purdue University
contact email	iokekeog@purdue.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD007612

PRIDE project URI

• PRIDE
  1. PXD007612
     1. Label: PRIDE project
     2. Name: Maize and Arabidopsis Golgi proteome