PXD007600

PXD007600 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis diurnal LFQ proteome and labelled phosphoproteome
Description	Plant growth and survival depends to a large extent on the diurnal regulation of cellular processes. Although extensively studied at the transcript level, notably less is known about diurnal fluctuations at the protein level. Here, we report a high-resolution quantitative time-course of the Arabidopsis rosette proteome and phosphoproteome over a 12 h light:12 h dark diel cycle. We monitored the proteome every 2 h and the phosphoproteome immediately before and after the light-to-dark and dark-to-light transitions. Notably, we quantified nearly 5000 proteins and 1800 phosphopeptides, of which 288 and 225, respectively, were found to fluctuate over the time-course. Diurnal proteome and phosphoproteome changes were related to diverse biological processes, including protein translation, light detection, photosynthesis, metabolism and transport. Together, these datasets represent the most comprehensive proteomic analysis of Arabidopsis rosettes to date, allowing us to make multi-level inferences about the diurnal regulation of key cellular plant processes plants.
HostingRepository	PRIDE
AnnounceDate	2020-12-18
AnnouncementXML	Submission_2020-12-18_03:58:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sira Echevarria-Zomeno
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-08-30 06:51:52	ID requested
⏵ 1	2020-12-18 03:58:35	announced

Uhrig RG, Echevarr, í, a-Zome, ñ, o S, Schlapfer P, Grossmann J, Roschitzki B, Koerber N, Fiorani F, Gruissem W, Diurnal dynamics of the Arabidopsis rosette proteome and phosphoproteome. Plant Cell Environ, 44(3):821-841(2021) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis, diurnal, proteome, phosphoproteome

Prof. Wilhelm Gruissem
contact affiliation	Department of Biology, Institute of Molecular Plant Biology, Plant Biotechnology, ETH Zurich
contact email	wgruisse@ethz.ch
lab head
Sira Echevarria-Zomeno
contact affiliation	Plant Biotechnology, ETH Zurich
contact email	esira@ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD007600

PRIDE project URI

• PRIDE
  1. PXD007600
     1. Label: PRIDE project
     2. Name: Arabidopsis diurnal LFQ proteome and labelled phosphoproteome